一种新型鸭瘟病原的分离鉴定及其特性  

Isolation and Identification and Characteristics of the Pathogen of a New Duck Plague

作　　者：刁有祥[1] 吕桂霞[1] 郑福英[1] 杨杰华[1] 陈庆普 刘悦竹 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]山东明发兽药研究所,山东济南250022

出　　处：《中国兽医学报》2006年第2期136-139,共4页Chinese Journal of Veterinary Science

基　　金：山东省自然科学基金资助项目(Y2001D05)

摘　　要：从2000年起．山东省的潍坊、临朐、昌乐、昌邑、沂源等饲养肉鸭集中的地区发生一种同鸭瘟症状、剖检变化相似的疾病．但该病主要侵害1月龄以下的雏鸭．成年鸭发病率较低。从自然感染该病典型病死鸭脏器中获得1株病毒，病毒粒子直径80～200nm，呈圆形．有囊膜。进一步试验鉴定，该病毒核酸类型为DNA,ELD50为10^-3.46／0．2mL，对樱桃谷鸭胚、番鸭胚、麻鸭胚及SPF鸡胚的致死率分别为100％、90％、20%和0％。该病毒对氯仿、乙醚、酸碱处理敏感，56℃ 30min能使病毒灭活，该病毒无血凝活性．不能凝集“O”型人、鸡、鸭、鹅、猪、小鼠、豚鼠、绵羊等的红细胞。血清学试验表明，该病毒与鸭肝炎病毒阳性血清、雏番鸭细小病毒阳性血清、小鹅瘟阳性血清之间无中和作用，而能部分中和鸭瘟病毒阳性血清，表明该分离株与传统鸭瘟病毒呈部分相关性。初步确定该病毒为疱疹病毒属疱疹病毒科成员。鉴于该病用传统的鸭瘟疫苗不能预防．故现暂定名为“新型鸭瘟”。From 2000,there is a new kind of meat duck disease take place in different districts of Shandong province. The symptom and pathological change of the disease is as same as duck plague,but mainly occurred in duckling,while adult duck less infected. A virus with 80-200 nm diameters and having envelope was isolated, from died meat duck. It＇s characteristics were also studied. The results showed that nuclear acid type of the virus was DNA. The ELD50 was 10^-3.46/0.2 mL. The mortality of the virus to cherry valley duck embryo,muscovy embryo,sheldrake embryo and SPF chicken embryo was 100% ,90%,20% and 0% respectively. The virus was sensitive to chloroform,aether,acid and alkali. When heated at 56℃ for 30 minutes,the virus will be inactived. The results of coaggulation test showed that the virus has no coaggulate properties to red blood cell. The results of serological test showed that the virus can not be neutralized by the serum of duck virus hepatitis ,gosling plague and muscovy duckling parvovirus disease. But it can be partially neutralized by duck plague serum. The results showed that the virus has partial relativity with duck plague virus. Through above tests,we confirmed that the virus was one of herpetoviridae, because it can not be neutralized by duck plague serum completely,so it is named tentatively a new type of duck plague virus.

关 键 词：鸭瘟 病原 新型鸭瘟病毒 

分 类 号：S852.65[农业科学—基础兽医学]