神经肽Y免疫反应神经元在鸽小脑中的定位——SABC法研究  

Localization of Neuropeptide Y Immunoreactive Neurons in Pigeon Cerebellum by Using SABC Method

作　　者：陈文钦[1] 刘华珍[1] 罗冠中[1] 王岩[1] 位兰[1] 彭克美[1] 

机构地区：[1]华中农业大学动物科技-动物医学学院,湖北武汉430070

出　　处：《中国兽医学报》2006年第2期180-183,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(39970547)

摘　　要：采用石蜡切片和免疫组化SABC法(链霉亲合素-生物素-过氧化物酶连结法),对10只肉鸽小脑内神经肽Y免疫反应神经元的分布状况进行了研究,并与北京鸭、乌鸡、肉鸡及大鼠的相关结果进行了比较。在光镜下观察分析了阳性神经元的分布状况,并用图像分析软件进行了半定量分析。结果显示:(1)小脑内神经肽Y(neuropeptide Y,NPY)阳性神经元主要存在于小脑皮质的蒲肯野氏细胞层,且以小叶顶端的蒲肯野氏细胞阳性明显;(2)小脑白质中央核可见到少量阳性神经元,不同于乌鸡、肉鸡及大鼠;(3)分子层、颗粒层未见阳性反应细胞。表明:鸽小脑NPY阳性神经元的分布在皮质中与鸡、大鼠大体相似;在白质中与鸡、大鼠有差异。In order to investigate the distribution of neuropeptide Y immunoreactive neurons in pigeon cerebellum,the cerebellum of ten meat pigeons were studied by paraffin consecutive sections and the immunohistochemical SABC staining method （Strept Avidin-Biotin complex）,and compared with that of Pekin duck, silky fowl. meat chick, rat. The distribution of neuropeptide Y immunoreactive neurons were observed under the light microscope ,and analyzed by a computer-assisted image analysis system. The results show as follows ： （1）immunoreactive neurons of NPY in pigeon cerebellum are mainly located in the Purkinje cell layer of the cerebellar cortex,especially in the top of Purkinje cell in cerebellar folia;（2）a few neuropeptide Y immunoreactive neurons are observed in the central nuclei of cerebellar white matter,which is different from silky fowl,chick and rat; （3）none of neuropeptide Y immunoreactive neurons are found both in the molecular and granular layer. The results of the present study indicate that the localization of neuropeptide Y immunoreactive neurons in pigeon cerebellar cortex is similar to that of chick and rat,but ,in cerebellar white matter is different from theirs.

关 键 词：神经肽Y SABC法 小脑 肉鸽 

分 类 号：S852.1[农业科学—基础兽医学]