慢性无症状乙型肝炎病毒携带者外周血T细胞受体谱型分析  

Analysis of T cell receptor repertoire in patients with chronic asymptomatic hepatitis B virus carriers

作　　者：张光文[1] 姚新生[2] 马世武[1] 于乐成[1] 王战会[1] 侯金林[1] 

机构地区：[1]南方医科大学南方医院感染内科,广州510515 [2]南方医科大学分子免疫学研究所

出　　处：《解放军医学杂志》2006年第3期246-249,共4页Medical Journal of Chinese People's Liberation Army

摘　　要：目的分析慢性无症状乙型肝炎病毒携带者(AsC)外周血T细胞TCR-β链CDR3谱型的变化,了解AsC患者T淋巴细胞克隆性增生情况。方法利用逆转录-聚合酶链反应扩增T细胞受体(TCR)各BV家族CDR3区基因,利用谱型分析技术比较各家族CDR3长度分布,了解AsCT淋巴细胞克隆性增生情况。对单克隆或寡克隆性增生家族的PCR产物进行序列测定和氨基酸序列分析,进一步了解克隆增生T淋巴细胞的均一性。结果4名正常人的TCRCDR3谱型均呈现正态分布,而9例AsC中均存在不同BV家族的T细胞克隆性增生,CDR3区直接测序结果证实增生的T细胞克隆具有不同长度和序列的氨基酸序列。结论AsC外周血T淋巴细胞存在克隆性增生,且增生的T淋巴细胞不具有均一性。Objective To explore the clonality of the T cells and the role of cellular immunological pathogenesis in chronic asymptomatic HBV carriers （AsC） by TCR CDR3 size spectratyping and determining sequence. Methods The TCR CDR3 region genes of 24 BV families were amplified by utilizing inverse polymerase chain reaction （RT-PCR） technology, and the CDR3 size lengths of T cell receptor （TCR） β-chain were analyzed with Genescan technology for 4 healthy individuals and 9 AsCs. The clonality of T cells presumed by spectratyping was further confirmed by CDR3 sequencing. Results The CDR3 repertoire of 4 healthy individuals showed Gaussian distribution. The clonal expansions of T cell were observed in 8 out of 9 AsCs. The expanded T cells have different CDR3 sequences. Conclusion There is significantly clonal expansion in the compartment of the peripheral blood T lymphocyte in AsCs. The expanded T cells do not have homogenicity.

关 键 词：肝炎病毒 乙型 受体 抗原 T细胞 互补决定区 

分 类 号：R373.21[医药卫生—病原生物学]