小麦幼穗离体培养高效再生系统的建立  

Establishment of High Efficient Regeneration System for In vitro Culture of Young Spike in Wheat

作　　者：赵林姝[1] 刘录祥[1] 郑企成[1] 王晶[1] 郭会君[1] 赵世荣[1] 陈文华[1] 

机构地区：[1]中国农业科学院作物科学研究所农业部农业核技术与航天育种重点开放实验室,北京100081

出　　处：《农业生物技术学报》2006年第1期96-101,共6页Journal of Agricultural Biotechnology

基　　金：国家高技术研究与发展计划(863)项目(No.2001AA212121);国际原子能机构跨地区项目(No.INT5147);国际原子能机构地区项目(No.RAS5040)资助

摘　　要：选用3个冬小麦(TriticumaestivumL)材料H6756、H311和SP8581,研究了不同取材时期、不同诱导培养基、分化培养基和生根培养基对小麦幼穗离体培养的影响。结果表明,在护颖原基形成期至雌雄蕊原基形成期之间对小麦幼穗进行离体培养都可获得较高的绿苗分化率,而在其它时期进行离体培养绿苗分化率均较低。确定了小麦幼穗离体培养的最佳诱导培养基为MS+2,4-D2mg/L,最佳分化培养基为MS培养基,并发现对分化过程中产生的变态苗增加一个芽苗的诱导及伸长阶段有助于继续分化成苗。最佳生根培养基为1/2MS+0.2mg/LIAA+80g/L蔗糖,在此基础上建立了小麦幼穗离体培养的高效再生系统。This study employed 3 winter wheat （Triticum aestivum L） genotypes H6756, H311 and SP8581 to compare the effect of sampling time, callus induction media, differentiation media and rooting media on young spike/n vitro culture in wheat. High frequencies of all the three kinds of green plantlet differentiation were obtained when their young spikes were cultured between the formation stage of protective glume primordium and formation stage of pistil and stamen primordium, but low before the formation stage of protective glume primordium and after the formation stage of pistil and stamen primordium. The optimum medium for callus induction was MS＋2,4-D 2 mg/L. The optimum green plantlet differentiation medium was MS. To those calli on which abnormal plants generated, adding a culture process of green plant induction and elongation after the differentiation culture could help them keep on differentiating and develop complete plants at last. The optimum rooting medium was 1/2MS＋0.2 mg/L IAA＋80 g/L sucrose. An efficient regeneration system for young spike culture of wheat was set up.

关 键 词：小麦 幼穗 离体培养 高效再生系统 

分 类 号：S181[农业科学—农业基础科学] S188