凡纳滨对虾白斑综合征血液病理研究  

Haemolymph pathological research on white spot syndrome(WSS) of Litopenaeus vannamei

作　　者：冯守明[1] 杨先乐[2] 李军[1] 王蓉[1] 赵燕超[1] 

机构地区：[1]天津市水产研究所,天津300221 [2]上海水产大学生命科学与技术学院,上海200090

出　　处：《水产学报》2006年第1期108-112,共5页Journal of Fisheries of China

基　　金：农业部丰收计划项目(200031231101)

摘　　要：对自然发病、投喂和注射感染的凡纳滨对虾白斑综合征(whitespotsyndrome,WSS)血液病理进行研究,结果发现:不同感染方式患病对虾的血液病理变化相似,表现为:1.患病对虾血细胞总数、透明细胞数量极显著减少,小颗粒细胞、大颗粒细胞极显著增加。2.显微病理变化主要表现为血涂片中血细胞明显减少且分布不均匀,破损或解体的细胞增多,呈典型的溶血状态。3.超微病理变化表现为,大部分血细胞坏死,少数血细胞呈不典型的凋亡。患病对虾的血细胞核中可见大量白斑综合征病毒(whitespotsyndromevirus,WSSV)粒子。病理变化表明血细胞是WSSV的主要靶细胞。The results of pathological research in haemolymph on WSS of Litopenaeus vannamei are as follows： pathological changes in WSS are evident and similar among diseased shrimps by different infecting manner. First, total haemocyte count in diseased shrimps is lower heavily than that in healthy shrimps. The percentage of the hyaline cell in diseased shrimps is lower heavily than in healthy shrimps. The percentages of small and large granule haemocytes of diseased shrimps are all higher heavily than those in healthy shrimps. Second, the main micro-pathological changes of haemocyte showed that there was an abnormal distribution of hemocytes in haemolymph smear from diseased shrimps. Broken and disintegrated cells can often be seen in the haemolymph smear. Third, the ultla-micro-pathological changes showed that there were necrotic changes in every kind of haemocytes, and there were atypical apoptosis phenomena in certain hemocytes; There were also many WSSV in certain hemocyte nucleus. All above showed that hemocyte cell was the main target cell of WSSV.

关 键 词：凡纳滨对虾 血液病理 白斑综合征 

分 类 号：S941[农业科学—水产养殖]