天然抗角蛋白自身抗体产生细胞的检测  

Detection of Natural Anti-keratin Autoantibody Producing Cells

作　　者：邢影[1] 李巍[1] 付萌[1] 田蓉[1] 韩骅[2] 王刚[1] 刘玉峰[1] 

机构地区：[1]第四军医大学西京医院皮肤科,陕西西安710032 [2]第四军医大学遗传与发育生物学教研室,陕西西安710032

出　　处：《中国皮肤性病学杂志》2006年第3期138-140,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金项目(30330510;30300318);军队重点基金项目(01Z086)

摘　　要：目的明确产生天然抗角蛋白自身抗体(anti-keratin autoantibody,AK auto Ab)的B细胞的类群和定位。方法取SPF级C57BL/6小鼠的腹腔细胞及脾细胞,进行体外培养,采用ELISA方法分析培养细胞分泌AK auto Ab的水平,ELISPOT方法分析分泌AK auto Ab的细胞数。结果腹腔细胞中分泌AK auto Ab的细胞数远多于脾细胞,其分泌的抗体滴度也显著高于脾细胞。结论AK auto Ab主要来源于腹腔B细胞,B1细胞可能是产生AK auto Ab的主要细胞。Objective To clarify the subsets and location of B cells producing natural anti-keratin autoantibody （anti - keratin autoantibody,AK auto Ab）. Methods Cells from spleen and peritoneal cavity of C57BL/6 mice were cultured in vitro, and ELISPOT was utilized to detect and calculate the cells that can identify keratin, and ELISA was applied to measure anti-keratin IgM level in culture supernatant. Results Number of Cells producing anti-keratin antibody in peritoneal cavity are greatly higher than those in spleen. And anti-keratin antibody level in the supernatant of cultured peritoneal cell was much higher than that of spleen cells. Conclusion Anti-keraitn autoantibody mainly originate from peritoneal cavity B cells, and B1 cells in the peritoneal cavity maybe the main source of anti-keraitn autoantibody.

关 键 词：角蛋白 天然抗角蛋白自身抗体 ELISPOT 

分 类 号：R392.11[医药卫生—免疫学]