夜蛾斯氏线虫对松树上松墨天牛的毒力测定和室内防治效果  

Biological Control of Monochamus alternatus by Steinernema feltiae

作　　者：胡学难[1] 陈小帆[2] 阮乐秋[2] 荣晓东[1] 吴佳教[1] 梁帆[1] 

机构地区：[1]广州出入境检验检疫局,广州510623 [2]广东出入境检验检疫局

出　　处：《中国生物防治》2006年第1期45-48,共4页Chinese Journal of Biological Control

基　　金：广东省科技厅科技计划项目(2202C1041001;2003B31402)

摘　　要：在室内测定了夜蛾斯氏线虫对松枝中松墨天牛的致死中量和致死中时。将TDM模型引入试验结果的分析中，72h后松墨天牛老龄幼虫的LD50为1．2767条／头，LB50为1．6716d；蛹的LD50为1．4417条／头，LB0为1．2627d。结果表明：随着时问的延长，LD50逐渐减少；随着线虫剂量的增加，LT50逐渐缩短。通过线虫寻向移动速度试验得出：夜蛾斯氏线虫与松墨天牛幼虫相距4—10cm情况下的效果较好。通过不同防治方法试验得出：施入夜蛾斯氏线虫防治松墨天牛，12d后，浸泡法的效果最好，海绵法次之，注射法较差。Monochamus alternatus is an important pest in wooden packing. In order to use Steinernema feltiae for control of M. alternatus in quarantine treatment, experiments were done to determine the LD50 and LT50 of M. alternatus by S. feltiae. Analysis showed that, for older larva, TDM （Time-dosemortality model） was Pil = 1 - exp [ 2.9957 ＋ 0.07471g （di） ], LD50 was 1.2767 nematodes per M. alternatus, and LT50 was 1.6716 d; for pupa, TDM was Pil = 1 - exp[ - 3.7567 ＋ 0. 23771g （di ）], LD50 was 1.4417 nematodes per M. alternatus, LT50 was 1.2627 d. Results showed that LD50 decreased when the test time extended, and LT50 shortened when dosage of S. feltiae increased. Host searching efficiency analysis revealed that M. alternatus could easily find S. feltiae when they were 4- 10 cm away. Control efficacy of S. feltiae against M. alternatus by different application methods of S. feltiae ranked as soaking, sponge and injection.

关 键 词：夜蛾斯氏线虫 松墨天牛 致死中量 致死中时 生物防治 

分 类 号：S763.38[农业科学—森林保护学] S476.15[农业科学—林学]