铜绿假单胞菌ZJ1999对水稻纹枯病的防治及其在水稻上的定殖  

Application and Colonization ofPseudomonas aeruginosa ZJ1999 for Biocontrol ofRhizoctonia solani, Pathogen of Rice Sheath Blight

作　　者：任小平 谢关林[2] 王笑[2] 

机构地区：[1]广东省植物检疫站,广州510500 [2]浙江大学生物技术研究所,杭州310029

出　　处：《中国生物防治》2006年第1期54-57,共4页Chinese Journal of Biological Control

基　　金：国家自然科学基金(30270891);亚洲开发银行资助项目

摘　　要：利用拮抗菌铜绿假单胞菌ZJ1999产生的粗提液进行防治水稻纹枯病研究的结果表明，抑制病菌侵染的活力随着粗提液浓度提高和处理时间的延长而增强。该菌株的定殖时间与最初引进时的浓度密切相关，其最低应用浓度为10^8cfu／ml，在接种纹枯病病菌后第一天的喷施防效最佳。拮抗菌ZJ1999在发病的秧苗、成株期植株茎杆和叶片上，14d后的菌量分别维持在10^2-10^3和10^3～10^4cfu／g。The crude extracts from the antagonistic bacterium Pseudomonas aeruginosa ZJ1999 were tested for effectiveness against Rhizoctonia solani, pathogen of rice sheath blight. Results showed that the inhibition ability against R. solani varied with concentration of tile extracts and the treatment time for mycelium of R. solani. The colonization duration of the antagonistic bacterium on rice plants was closely related to the initial concentration. The population of P. aeruginosa ZJ1999 could retain at 10^2 - 10^3 cfu/g and 10^3 - 10^4 cfu/g 14 d .after spraying on seedling and adult plants. Minimum concentration of the extract was 10^8 cfu/ml, and the optimum spraying time was at the first day after inoculation of R. solan for best control of rice sheath blight.

关 键 词：水稻纹枯病 铜绿假单胞菌 定殖 生物防治 

分 类 号：S435.111.42[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]