上海市吴淞工业区大气PM_(2.5)水溶成分的元素分析及细胞毒性研究  

Studies on elemental components and cytotoxicity of water-soluble constituents in PM_(2.5) collected in Wusong Industrial Area

作　　者：程硕[1] 王伟[1] 谈明光[1] 陈建敏[1] 张桂林[1] 李燕[1] 

机构地区：[1]中国科学院上海应用物理研究所,上海201800

出　　处：《核技术》2006年第3期182-188,共7页Nuclear Techniques

基　　金：中国科学院知识创新工程项目(KJCXZ-SN-NO1);国家基金委十五重大项目;上海市自然科学基金项目(O3ZR14111)

摘　　要：通过ICP-MS测定了上海市吴淞地区大气PM2.5水溶成分金属元素含量,通过四唑盐(MTT)测定细胞存活率,通过超氧化歧化酶(SOD)和丙二醛(MDA)两个指标测定细胞氧化损伤,琼脂糖凝胶电泳测定细胞内DNA损伤,流式细胞仪(Flowcytometry)检测细胞凋亡和周期等实验研究了大气PM2.5水溶成分的细胞毒性。实验结果表明:PM2.5可溶成分中Zn元素含量最高,PM2.5在一定浓度范围内能导致细胞死亡,引起细胞的氧化损伤,影响DNA的复制,阻碍细胞增殖,但没有诱导细胞凋亡。PM2.5中可溶金属离子对细胞毒性表现为联合作用。The elemental concentration of water-soluble component of PM2.5 collected in Wusong Industrial Area, Shanghai was measured by ICP-MS. Studies on the cytotoxicities of such component were carried out. The rate of cell proliferation was detected by MTT assay. Both SOD and MDA assays were adopted for assessing the ability to cause cell oxidative damage. Agar gel electrophoresis and FCM were used to examine the cellular DNA damage. The results show that the water-soluble metal with the highest concentration in PM2.5 is zinc. Our data demonstrate that the water-soluble component of PM2.5 can mediate cell proliferation, cause cell oxidative damage and attenuate the cell DNA synthesis, but can not cause cell apoptosis action. In conclusion, these water soluble metal ions in PM2.5 displayed the synergic effects.

关 键 词：大气颗粒物 PM2.5 氧化损伤 细胞凋亡 锌离子 

分 类 号：X5[环境科学与工程—环境工程] Q2[生物学—细胞生物学]