氯磺酚M与血清蛋白质相互作用的光度研究及分析应用  

Spectrophotometric study on the interaction between proteins and chlorosulfophenol M and its analytical application

作　　者：胡秋娈[1] 

机构地区：[1]洛阳师范学院化学系,河南洛阳471022

出　　处：《化学试剂》2006年第3期153-155,共3页Chemical Reagents

基　　金：河南省自然科学基金资助项目(0411021600)

摘　　要：用于测定稀土等无机离子的显色剂氯磺酚M（chlorosulfophenol M），在pH4．23的Bfitton-Robinson（B-R）缓冲介质中，能与生物活性物质血清蛋白质形成有色复合物，λmax为615nm，比试剂本身红移约63nm，表观摩尔吸光系数ε为4．34×10^5L·mol^-1·cm^-1，测定蛋白质的线性范围为0～100mg/L（牛血清白蛋白，BSA）。应用于临床中人血清样品总蛋白质的测定，结果与经典方法一致，而且操作简便、线性范围宽，重现性好，基本无干扰。Chlorosulfophenol M is a colouring reagent for the determination of some inorganic ions. It was found that chlorosulfophenol M could bind with serum proteins to form complex in Britton-Robinson buffer at pH 4.23, which presents a maximum absorption at 615nm with 63nm of red shift compared to chlorosulfophenol M itself. A molar absorptivity of4.34×10^5L·mol^-1·cm^-1（BSA） and linear range of 0-100mg/L for protein were determined. This method has been used for the determination of the total protein in human serum samples. The results obtained by this method agreed well with those obtained by coomassie briliant blue G-250 method. Furthermore, the performance and reproducibility are superior to coornassie brilliant blue G-250 method.

关 键 词：吸光光度法 氯磺酚M 血清蛋白质 

分 类 号：O657.3[理学—分析化学]