混胺(NH_3/CH_3NH_2)草酸根合铂(Ⅱ)混配配合物对人体癌细胞的作用及机制  

Effect on the human cancer cells and anticancer mechanism of platinum(Ⅱ) complex of ammine/amine(NH_3/CH_3NH_2) with oxalate

作　　者：张金超[1] 张祥[2] 龚钰秋[1] 郑小明[1] 崔景荣[2] 

机构地区：[1]浙江大学化学系 [2]保定中法供水有限公司,河北保定071000

出　　处：《浙江大学学报（理学版）》2006年第2期200-203,208,共5页Journal of Zhejiang University（Science Edition）

基　　金：浙江省自然科学基金资助项目(298068);北京大学医学部天然药物及仿生药物国家重点实验室资助项目

摘　　要：采用MTT和SRB法研究了混胺(NH3/CH3NH2)草酸根合铂(Ⅱ)混配配合物(MAOX)对人体癌细胞的增殖抑制作用,又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制.实验结果表明:在10μmol.L-1浓度下,该配合物对HL-60和EJ两种细胞有较高的活性,其抑制率分别为51.35%和45.86%;它能阻止HL-60细胞G2+M→G1期的进行,造成G2+M期细胞的堆积;对HL-60细胞的凋亡诱导作用不明显;在相同浓度情况下其与HL-60细胞的DNA键合量大于顺铂.The antitumor activity of platinum（Ⅱ） complex of ammine/aminc （NH3/CH3NH2） with oxalate was determined by MTT and SRB methods in vitro, At the same time, the anticancer mechanism was studied by flow cytometry, Giemsa and Icp-Ms. The experimental results indicated that the complex had fairly higher activity against HI. 60 and EJ, and the inhibition rate was 51.35 and 45.86% at concentration of 10 μmol·L^-1 , respectively. The cell cycle was arrested on the G2＋M→G1 , the effect of the complex on apoptosis was not obvious, and the level of total platinum bound to DNA was higher than that of cisplatin under the same conditions.

关 键 词：混胺铂配合物 抗肿瘤活性 细胞周期 凋亡 DNA 

分 类 号：O624.121[理学—有机化学]