唾液酸相关物质对盐酸致伤大鼠气管铜绿假单胞菌感染的抑制作用  

Inhibition of sialic acid related substances on Pseudomonas aeruginosa infection in rat trachea vulnerated by hydrochloric acid

作　　者：黄勇[1] 钱桂生[1] 徐顺贵[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸内科研究所

出　　处：《第三军医大学学报》2006年第5期462-464,共3页Journal of Third Military Medical University

摘　　要：目的探讨唾液酸双糖、特异性植物凝集素及唾液酸酶对盐酸致伤大鼠气管铜绿假单胞菌感染的抑制作用。方法大鼠气管内滴入盐酸致伤,接种分别含唾液酸双糖(唾液酸α2,3半乳糖、唾液酸α2,6N乙酰氨基半乳糖)、特异性植物凝集素(Maackiaamurensisagglutinin,MAA;Sambucusnigraagglutinin,SNA)及唾液酸酶(特异性α2,3唾液酸酶和非特异性唾液酸酶)的铜绿假单胞菌混合液,分别在30min和48h后处死动物,完整取气管称重、匀浆后做细菌定量培养。结果唾液酸α2,3结构的双糖、凝集素和唾液酸酶都能明显减少铜绿假单胞菌接种30min后气管粘附的菌落数,而只有双糖和唾液酸酶能显著减少接种48h后气管的菌落数;唾液酸α2,6结构的相关物质没有类似的作用。结论唾液酸α2,3结构相关物质能干预铜绿假单胞菌在气管的粘附/繁殖,双糖和唾液酸酶的作用更明显。Objective To investigate whether sialic acid disaccharides, specificity agglutinins and neuramidinases could inhibit Pseudomonas aeruginose infection in rat trachea vulnerated by hydrochloric acid. Methods One hundred and forty male Wistar rats were vulnerated by hydrochloric acid through intratracheal instillation and then inoculated by Pseudomonas aeruginose admixed Sial α2,3-Gal, Sial α2,6-GalNAc, Maackia amurensis agglutinin, Sambucus nigra agglutinin, specificity α2,3-neuramidinase or non-specificity neuramidinase respectively. Thirty minutes and 48 h after inoculation, whole trachea of 8 rats in each group was weighed, homogenated, and quantitative bacterial culture was performed while whole trachea of another 2 was fixed in 10% methanol, embedded with paraffin and HE stained. Results Sial α2,3-related disaccharide, agglutinin and neuramidinases could decrease colony count of trachea 30 min after inoculation remarkably. But only Sial α2,3-Gal and neuramidinases could obviously decrease colony count 48 h later. Sial α2,6-related substrances have not analogical effects. Conclusion Sial α2,3-related substrances could interfere Pseudomonas aeruginose binding/multiplication in the trachea, especially Sial α2,3-Gal and neuramidinase.

关 键 词：唾液酸相关物质 大鼠 气管 铜绿假单胞菌感染 

分 类 号：R63[医药卫生—外科学] R643[医药卫生—临床医学]