泡状棘球蚴感染对大鼠免疫学状态及移植心存活的影响  

Immunology state and the allograft heart survival in Echinococcus multilocularis(Em) infected rats

作　　者：邵英梅[1] 赵晋明[1] 温浩[1] 陈实[2] 

机构地区：[1]新疆医科大学第一附属医院普外科,乌鲁木齐830000 [2]华中科技大学同济医学院附属同济医院器官移植研究所

出　　处：《中华器官移植杂志》2006年第3期132-134,共3页Chinese Journal of Organ Transplantation

摘　　要：目的研究肝泡状棘球蚴感染对大鼠免疫学状态及移植心存活的影响。方法建立SD大鼠到W istar大鼠的颈部异位心脏移植模型。对照组以未接种泡状棘球蚴的W istar大鼠为受者;实验组以感染泡状棘球蚴的W istar大鼠为受者。术后观察移植心的存活时间、组织病理学变化、心肌组织内T淋巴细胞和嗜酸粒细胞的浸润情况,测定血清内白细胞介素4(IL-4)和γ干扰素(IFN-γ)水平。结果实验组移植心的存活时间与对照组相比,差异有统计学意义(P<0.05),组织病理学分级及心肌组织中CD4+T淋巴细胞数与对照组相比,差异无统计学意义,而CD8+T淋巴细胞数、嗜酸粒细胞数与对照组相比,差异均有统计学意义。发生排斥反应时,实验组血清内IL-4水平高于对照组,而IFN-γ水平低于对照组。结论泡状棘球蚴感染造成TH1/TH2向TH2类细胞因子偏移,有利于移植物的存活,嗜酸粒细胞浸润可能是移植心发生排斥反应的原因之一。Objective To study the influence of echinococcus muhilocularis （Em） infection on the immunology state and the allograft heart survival in the infected rats. Methods SD-to-Wistar allograft heart transplantation models were established to observe the influence of the disturbed immune system in Em infected rat on the heart graft. Sixteen healthy SD rats, 8 healthy Wistar rats and 8 Em infected rats were used in the study. Animals were randomly divided into： （1） control group：SD- to-healthy Wistar rats （n = 8） and （2） Em infection group： SD-to-Em infected Wistar rat （n = 8）. The allograft heart transplantation was performed by anastomosis of the donor heart of SD rats into the cervices of the Wistar rats. All the agents were administrated orally q. d. Graft samples were harvested for histopathological examination and immunohistochemical study. IL-4 and IFN-γ in peripheral blood were assessed. CD4^＋ ,CD8^＋ and CD15Tc infiltration in the graft was detected. Results The mean survival time of the grafts in Em infected rats was 11.63 ±6. 02 days and 6. 00 ±. 53 days in no-infected rats respectively with the difference being significant （P〈0. 05）. Histopathological stage and CD4^＋ T- lymphocytes infiltration in showed no significant difference between infected Em group and control group, but there was significant difference in infiltration of CD8^＋ T-ymphocytes and eosinophils. On the other hand, level of serum IL-4 in infected Em group was higher than that in control group, but level of serum IFN-γwas lower when rejection occurred in grafts. Conclusions Em infection can induce the deviation of the Th2 type cytokines, which is beneficial to the survival of the grafts. Infiltration of the Eosinophils is the primary cause that leads the grafts to be rejected and failure.

关 键 词：心脏移植 棘球蚴病 肝 T淋巴细胞 嗜酸细胞 

分 类 号：R532.32[医药卫生—内科学] R654.2[医药卫生—临床医学]