骨髓间充质干细胞与嗅鞘细胞联合移植治疗脊髓损伤的早期观察  

Preliminary observation of stem cells and olfactory ensheathing cells graft for treatment of spinal cord injury

作　　者：赵廷宝[1] 卢兆桐[1] 赵凌云[1] 陈秉耀[2] 张云昌[1] 张晓君[1] 刘鑫[1] 孟庆溪[1] 林世德[1] 

机构地区：[1]济南军区总医院脊髓修复科,济南250031 [2]解放军总医院304临床部全军骨科中心,北京100036

出　　处：《中国矫形外科杂志》2006年第5期346-348,共3页Orthopedic Journal of China

摘　　要：[目的]探讨自体骨髓间充质细胞和异体嗅鞘细胞共培养及其联合移植修复脊髓损伤的可行性，为临床治疗提供一种新方法。[方法]无菌条件下采集自体骨髓间充质细胞和胎龄4～6个月的引产胎儿的嗅鞘细胞，分别培养至传代后再共培养，将共培养的混合细胞通过手术直视下移植至脊髓损伤部位。[结果]8例脊髓损伤患者接受自体骨髓间充质细胞和异体嗅鞘细胞联合移植，并获得1～6个月的随访，所有接受细胞移植的8例患者术后均无不良反应，其中1例术后1个月开始出现双下肢深浅感觉恢复，其余7例比细胞移植前无明显改善。[结论]自体骨髓间充质细胞和异体嗅鞘细胞共培养两者之间无相互抑制现象，近期观察二者联合移植修复脊髓损伤是安全的。[ Objective] To investigave the feasibility of commensalism of stem cells and olfactory ensheathing cells and to study the preliminary results of graft of stem cells and olfactory ensheathing cells for the treatment of spinal cord injury. [Method] Under the condition of sterility, the stem cells and olfactory ensheathing cells were collected from the embryon of induced labor for 4 to 6 months and the patients. The stem cells and olfactory ensheathing cells were cultured individually until transduced generation, then were cultured together and transplanted into the place where the spinal cord was injuried in the patient. [ Resalt] Eight patients who had the spinal cord injury were treated with the graft of stem cells and olfactory ensheathing cells and were followed up for 1 to 6 months after the operation. One case with the T5 -7 spinal cord injury got the recovery of superfical and deep sensation of the lower limbs. The other 7 patients had not obvious recovery. [ Conclusion ] There was no appearance of inhibition when the stem cells and olfactory ensheathing cells were cultured together. The united graft of the stem cells and olfactory ensheathing cells for the treatment of spinal cord injury is safe with the short-term observation.

关 键 词：骨髓间充质干细胞 嗅鞘细胞 移植 脊髓损伤 

分 类 号：R651.2[医药卫生—外科学]