低剂量镉处理后大鼠睾丸差异表达基因的cDNA微阵列分析  

cDNA Microarray Analysis in Testes of Rats Exposed to Low Cadmium Toxicity

作　　者：张建鹏[1] 任绪义[1] 郭婷婷[2] 冯伟华[1] 焦炳华[1] 

机构地区：[1]第二军医大学基础医学部生物化学与分子生物学教研室,上海200433 [2]上海水产大学生命科学与技术学院,上海200090

出　　处：《癌变．畸变．突变》2006年第2期84-87,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家自然科学基金(No.39970631)

摘　　要：背景与目的深入探讨睾丸镉毒性机制。材料与方法应用cDNA微阵列分析和实时定量PCR技术对低剂量镉处理后大鼠睾丸组织基因表达情况进行分析。结果UDP-葡萄糖醛酸转移酶、血红素加氧酶、错配修复蛋白、T-激肽原和cal megin等基因与镉毒性相关,但作为产能的细胞器,线粒体呼吸链并未受到低剂量镉影响。另显示维生素C对镉毒性有明显的缓解作用。结论镉诱导毒性和致癌作用的影响可能涉及能量代谢、防御保护、DNA修复多个方面;维生素C能够明显降低镉对大鼠睾丸的毒性作用。BACKGROUND ＆ AIM： To identify genes involved in cadmium-induced testieular toxicity. MATERIAL AND METHODS： We used eDNA mieroarray and real-time quantitative polymerase chain reaction（real-time PCR） technique to analyze the genes expression of testes in rats treated with low cadmium（4μmol/kg）. RESULTS： Our studies for the first time demonstrated that expression of T-kininogen, calmegin, UDP-glucuronyl transferase, heme oxygenase and mismatch repair protein gene were associated with cadmium toxicity. However, as the organdies that produced energy, mitochondrion was not affected in the respiratory chain at low dose cadmium. In addition, vitamin C at the concentration of 400 mg/kg obviously attenuated cadmimn-induced toxicity in testes of rats. CONCLUSION： The cadmimn-reduced toxicity carcinogenic effects involved energy metabolism, defense mechanisms and DNA repair of the cell. Vitamin C might play essential roles in protecting testes of rats exposed to cadmium.

关 键 词：大鼠毒理学芯片 定量RT-PCR 线粒体 维生素C 

分 类 号：R1[医药卫生—公共卫生与预防医学] Q7[生物学—分子生物学]