稀土镧离子对大肠杆菌基因组DNA的影响  

Effects of Lanthanide Ions in Rare Earths on Genomic DNA of E.coli

作　　者：汪承润[1] 陈华波[1] 杨帆[2] 郭汉卿[2] 王子尧[2] 丁铁林[2] 

机构地区：[1]淮南师范学院化学生物系,安徽淮南232001 [2]淮南师范学院化学生物系02级生命科学专业,安徽淮南232001

出　　处：《癌变．畸变．突变》2006年第2期116-118,共3页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：安徽省教育厅自然科学基金项目(No.2005KJ197)

摘　　要：背景与目的通过研究硝酸镧溶液对大肠杆菌基因组DNA的作用,探讨稀土镧离子对原核生物的遗传毒性效应。材料与方法用含硝酸镧分别为10、40、80、160、320mg/L的LB培养基培养大肠杆菌,以不添加硝酸镧组作为对照,于培养10h,15h和20h后分别采样提取总DNA,琼脂糖凝胶电泳;并用含硝酸镧分别为50、200、400、800、1600mg/L的LB培养基培养大肠杆菌,未添加硝酸镧组作为对照,于培养30h后分别采样提取总DNA,琼脂糖凝胶电泳。结果高剂量镧离子组大肠杆菌基因组DNA出现降解或交联现象,电泳带型荧光减弱,不清晰或滞留于点样孔不能泳出。结论镧离子对大肠杆菌基因组DNA可能具有降解或交联作用。BACKGROUND ＆ AIM： To investigate genotoxic effect of lanthanide ions on prokaryote by observing the effects of lanthanide nitrate on genomic DNA of E. coli. MATERIALS AND METHODS： Treatment Ⅰ ： E. coli was cultured in LB medium with different concentrations of lanthanide nitrate （10, 40, 80, 160, 320 mg/L）, LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 10, 15, and 20 h. Then Agarose gel electrophoresis was performed Treat ment Ⅱ ： E. coli was cultwred in LB medium with different concentrations of lanthanide nitrate （50,200,400,800, 1 600 mg/L） LB medium without lanthanide nitrate was the control. Genomic DNA was extracted at 30 h. Then Agarose gel electrophoresis was performed RESULTS： DNA degradation or cross-linkage was produced by high concentrations of lanthanide ions. CONCLUSION： Lanthanide ions affected degradation and crosslinking on genomic DNA of E. coli .

关 键 词：稀土 镧离子 大肠杆菌 基因组DNA DNA降解作用 DNA交联作用 

分 类 号：Q255[生物学—细胞生物学] Q503