小鼠毒理基因芯片的设计和制作  

The Design and Fabrication of Mouse Toxicology Gene Chip

作　　者：敖琳[1] 曾志雄[2] 方志俊[2] 胡冉[1] 高利宏[1] 杨梦苏[2] 曹佳[1] 

机构地区：[1]第三军医大学预防医学系卫生毒理学教研室,重庆400038 [2]香港城市大学基因组科技应用研究中心

出　　处：《癌变．畸变．突变》2006年第2期135-138,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：国家973课题分题(No.2002CB512901);国家杰出青年基金资助项目(No.30125037);国家自然科学基金资助项目(No.30271136;30200354)

摘　　要：背景与目的设计并制作一种用于毒理学实验研究的小鼠毒理基因芯片。材料与方法根据环境与毒理学研究的要求,挑选各个生物学通路上的相关基因,采用美国NIA(National Institute on Aging)小鼠cDNA文库克隆扩增所挑选的基因片段,点样法制作芯片,荧光染色法抽样检验芯片质量。结果设计的小鼠毒理基因芯片含1796个基因,涉及8种生物学功能;PCR扩增的目的基因片段95.12%为完全合格;Picogreen荧光染色实验可用于检验芯片的点样质量。结论目的基因扩增和芯片制作基本成功,自行研制的毒理基因芯片为进一步用于毒理学相关研究创造了条件。BACKGROUND ＆ AIM： To design and fabricate a kind of mouse toxicology gene chip to be used in toxicology research. MATERIAL AND METHODS： Some genes related to different biological functions were chosen according to the requirement of environmental and toxicology research. NIA mouse cDNA clone sets were applied to copy and amplify the gene fragments which were dotted on the slide, and the Picogreen fluorescence staining was used to detect the quality of chip. RESULTS： We chose 1 796 genes involved in eight biological functions for the construction of the meuse toxicology gene chip. 95.12% of the PCR products had strong and single band, and the slides selected from each hatch had good images with Picogreen dye detection. CONCLUSION： We have designed and fabricate the mouse toxicology, gene chip successfully. This could be applied in toxicology-related research.

关 键 词：基因芯片 毒理学 

分 类 号：R99[医药卫生—毒理学]