己烯雌酚对2、4、6-三硝基苯磺酸/乙醇诱导的大鼠结肠炎的影响  

Effect of diethylstilbestrol on TNBS-induced colitis

作　　者：金艳[1] 刘莉[1] 王庆伟[1] 王志鹏[1] 李宇华[1] 冯娟[1] 梅其炳[1] 

机构地区：[1]第四军医大学药学系药理教研室

出　　处：《中国临床药理学与治疗学》2006年第2期168-172,共5页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金资助项目(№30300450)

摘　　要：目的:观察己烯雌酚对2、4、6-三硝基苯磺酸(trinitrobenzene sulfonic,TNBS)/乙醇诱导的大鼠结肠炎的双向调节作用。方法:TNBS诱导大鼠结肠炎模型,肌肉注射己烯雌酚(50、100、150、200μg.kg-1),每天1次,共7 d。给药结束后,乙醚麻醉大鼠,打开腹腔,截取8 cm结肠,称重,画取溃疡面积,取病变组织以作HE染色,分离肠粘膜待测MPO活性;并取胸腺、脾脏称量。结果:50μg.kg-1己烯雌酚对结肠炎无明显治疗作用,反而引起肠道粘连和腹膜炎,加重炎症;100μg.kg-1的己烯雌酚对结肠炎具明显疗效;150和200μg.kg-1己烯雌酚能明显改善结肠炎各项指标,但大鼠全身状态差,死亡率增高。结论:适当剂量的己烯雌酚对结肠炎具有治疗作用,小剂量的己烯雌酚能够加重结肠炎的发生。AIM： To observe the modulatory action of diethylstilbestrol on TNBS-induced colitis. METHODS： After the rats with colitis induced by TNBS were injected intramuscularly with diethylstilbestrol at doses of 50,100, 150,200μg·kg^-1 respectively, they were killed on day 7 postdose. Then determined colon weight, ulcerative erea, thymus weight, spleen weight, MPO aetivity and observed microstructure with HE staining. RESULTS： Diethylstilbestrol at the dose of 50μg·kg^-1 deteriorate the inflammarion, but diethylstilbestrol of 100μg·kg^-1 amelioratethe disease. Interestingly, diethylstilbestrol increased the mortality rate of the rats though it relieved inflammatory response significantly at the doses of 150 and 200μg·kg^-1. CONCLUSIOND： Diethylstilbestrol exhibits curative effect at the proper dose, while much lower or higher level of diethylstibestrol can not be used to treat the colitis.

关 键 词：己烯雌酚 结肠炎 细胞因子 2、4、6-三硝 基苯磺酸 自身免疫 

分 类 号：R965[医药卫生—药理学]