葛根素对胰岛素抵抗大鼠肝脏中GSK-3表达的影响  

Effects of puerarin on GSK-3 in hepatic cell in rats with insulin resistance

作　　者：洪畋[1] 毕会民[1] 毕欣[1] 

机构地区：[1]武汉大学人民医院内分泌科

出　　处：《中国临床药理学与治疗学》2006年第2期207-210,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：湖北省自然科学基金资助课题(№2003ABA182)

摘　　要：目的:观察高糖高脂饮食诱导的胰岛素抵抗大鼠通过葛根素治疗后肝脏中GSK-3的表达。方法:将30只Wistar大鼠随机分为正常组、模型组和葛根素组,每组各10只。3组均以基础饲料喂养一周,然后正常组仍以基础饲料喂养,模型组和葛根素组改喂高糖高脂饮食,4周后葛根素组大鼠给予100mg.kg-1.d-1葛根素腹腔注射,连续6周,待造模成功后,用Western-blot法检测各组大鼠肝脏中GSK-3的表达,并分别在1、51、1周定期检测TG(总甘油三酯),TC(总胆固醇),FBG(空腹血糖),FINS(空腹胰岛素)并计算ISI(胰岛素敏感指数)和HOMA-IR(胰岛素抵抗指数)。结果:模型组大鼠肝脏中GSK-3的表达较正常组增高129.4%(P<0.01),用葛根素治疗后,大鼠肝脏中GSK-3的表达较模型组减少14.6%。结论:葛根素可降低胰岛素抵抗大鼠肝脏中GSK-3的表达。AIM： To develop a rat model of insulin resistance and observe the effect of puerarin injection on expression of GSK-3 in insulin resistance rats. METHEDS： 30 Wistar rats were divided into three groups randomly： control group, model group and puerarin group. The model group and puerarin group were given high fat diet （20% surcose, 10% ard, 2.5% chlesterol, 1% cholic acid） for 4 weeks. Then the puerarin group was given abdominal injection puerarin 100 mg·kg^-1·d^-1 for six weeks. At the end of this experiment, we detect GSK-3 protein by western-blot analysis. Then computerimage pattern analysis system was used to analyze the expression of GSK-3 in the hepatacyte. RESULTS： Tthe model group showed a hyperglycemia, hyperinsulinism, the ISI decreased and HOMA-IR increased . GSK-3 expression enhanced in the model group, but the expression of GSK-3 in puerarin group decreased compared to the model group. CONCLUSION： Puerarin injection can significantly decreased the expression of GSK-3 and improve insulin resistance.

关 键 词：葛根素 胰岛素抵抗 GSK-3 动物模型 肝脏 

分 类 号：R979.15[医药卫生—药品]