壳聚糖调节不结球白菜叶片GDH和GS基因的差异表达研究  

Chitosan Regulation to Differentiated Expression of GDHmRNA and GSmRNA in the Leaves of No-Heading Chinese Cabbage

作　　者：陈惠萍[1] 徐朗莱[1] 

机构地区：[1]南京农业大学生命科学学院

出　　处：《西北植物学报》2006年第3期507-511,共5页Acta Botanica Boreali-Occidentalia Sinica

摘　　要：应用半定量RT-PCR技术检测了壳聚糖对不结球白菜叶片GDH和G S的mRNA水平的影响。结果表明GDHmRNA的变化与GDH活性的变化趋势基本一致,而G SmRNA的量基本恒定,并未与G S的活性变化相符合。即壳聚糖可能在转录水平上对氨同化关键酶GDH进行调控;而对G S的调控则不在转录水平上,有可能发生在翻译水平上。Semi-quantitative RT-PCR was adopted to determine the effects of chitosan on the levels of GDHmRNA AND GSmRNA in the leaves of no-heading Chinese cabbage, and the results showed that GDHmRNA tended to vary basically in the same pattern as GDH activity did and GSmRNA remained nearly constant without consisting with GS activity, that is to say,chitosan probably regulate GDH ,the crucial enzyme to ammonia assimilation,at the transcriptional level,but it probably regulate GS at the translation level in steady of the transcriptional level.

关 键 词：壳聚糖 不结球白菜 GDHmRNA GSmRNA RT—PCR 

分 类 号：Q786[生物学—分子生物学]