蓝浆果叶片高效再生体系的建立  

Construction of the High-efficiency Regeneration System of Blueberry with Its Leaves

作　　者：陶建敏[1] 耿其芳[1] 庄智敏[1] 刘捷[1] 章镇[1] 蔡斌华[1] 

机构地区：[1]南京农业大学园艺学院,南京210095

出　　处：《西北植物学报》2006年第3期610-614,共5页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家农业综合开发科技示范项目[财发(2001)14];国家863项目(P200310)

摘　　要：以高丛蓝浆果（Vaccinium corymbosum）叶片为外植体，研究了影响离体再生的多个因素，建立了高效的再生体系。结果表明：改良1／2MS＋ZR5mg·L^-1培养基最适合不定芽分化，再生率达100％。不同节位叶片对蓝浆果叶片不定芽的分化具显著效应，以幼嫩的1～2节位的叶片比较容易分化不定芽；叶片远轴面接触培养基、叶片创造伤口和不经暗处理或短时间处理有利于不定芽分化。低浓度IBA有利于叶片再生茎段的生根，在改良1／2MS＋IBA0．1mg·L^-1培养基上蓝丰（Bluecrop）生根率为66．7％，而伯克利（Berkly）仅为33．3％。Vacciniurn coryrnbosurn leaves were used as the explants to study quiet a few factors affecting in vitro regeneration and construct its high-efficiency regeneration system. The results indicated that the modified 1/2 MS with 5 mg · L^-1 Zeatin （t-ZR） was the optimal medium for adventitious bud differentiation and its regeneration rate reached as high as 100%. The leaves at different nods showed significant effects on adventitious-bud differentiation of blueberry leaves and the leaves on the first-second nodes were liable to differentiate adventitious buds;Medium contacts of distal leaf surface, artificial leaf wounds, and nildarkness or short-time dark treatments were conductive to adventitious bud differentiation. IBA at low concentration are favorable for leaf-regenerated stems to root and on the modified 1/2 MS with 0.1 mg · L^-1 IBA,the rooting rate of bluecrop was 66.7% whereas that of berkly was 33.3%.

关 键 词：蓝浆果 不定芽再生 叶位 生根 

分 类 号：Q813.1[生物学—生物工程] Q949.772.3