低温在大鼠脑缺血实验中的保护作用  

Protection effect of hypothermia used in ischemia reperfusion model in rats

作　　者：韩非[1] 李文志[1] 王红蕾[1] 

机构地区：[1]哈尔滨医科大学附属第二医院麻醉科,150086

出　　处：《国际麻醉学与复苏杂志》2006年第1期16-18,共3页International Journal of Anesthesiology and Resuscitation

摘　　要：Objective To evaluate the influence of hypothermia to Ca 2+/calmodulin-dependent kinase Ⅱ during global brain ischemia in rats. Methods Fifteen male SD rats were randomly divided into 3 groups: control group (group Ⅰ, n=5), hepothermia group (group Ⅱ, n=5) and reperfusion group (group Ⅲ, n=5). Only sugery was performed without hypothermia and clipping bilateral carotids in group Ⅰ. Ice was used covering head of rats in group Ⅱ to get hypothermia around 32℃, and bilateral carotids were clipped 20 min of ischemia followed by 60 min reperfusion keeping tympanum temperature 32℃. Bilateral carotids were clipped 20 min of ischemia followed by 60 min reperfusion without hypothermia in Group Ⅲ. Brain tissues were saved in three groups after experiments for evaluating Ca 2+/calmodulin-dependent kinase Ⅱ. Results Left tympanum temperature in the group Ⅱ is significantly lower than group Ⅰ during global ischemia and reperfusion(p<0.05). Those are no difference between group Ⅰ and group Ⅲ. The activity of Ca 2+/calmodulin-dependent kinase Ⅱ after 60 min reperfusion in both Group Ⅱ and group Ⅲ was significantly lower than those in the group Ⅰ(P<0.05), which is significantly higher in the group Ⅱ than in the group Ⅲ(P<0.05). Conclusion Hypothermia can increase the activity of Ca 2+/calmodulin-dependent kinase Ⅱ after global ischemia reperfusion. Hypothermia has significant protection during ischemia reperfusion injury.Objective To evaluate the influemce of hypothermia to Ca^2＋/calmodulin -dependent kinase Ⅱ during global brain isehemia in rats. Methods Fifteen male SD rats were randomly divided into 3 groups： control group （group Ⅰ, n =5）, hepothemia group （group Ⅱ, n ： 5 ） and reperfusion group （group Ⅲ, n =5）. Only sugery was performed without hypothermia and clipping bilateral carotids in group Ⅰ, Ice was used covering head of rats in group Ⅱ to get hypothemia around 32℃, and bilateral carotids were clipped 20 rain of ischemia followed by 60 min reperfusion keeping tympanum temperature 32℃. Bilateral carotids were clipped 20 min of isehemia followed by 60 min reperfusion without hypothernfia in Group Ⅲ. Brain tissues were saved in three groups after experiments for cvaluating Ca^2＋/calmodulin - dependent kinase Ⅱ. Results left tympauum temperature in the group Ⅱ is significantly lower than group Ⅰ during global ischemia and reperfusion（p 〈0.05）. Those are no difference between group Ⅰ and group Ⅲ. The activity of Ca^2＋/calmodulin - dependent kinase Ⅱ after 60 min repeffusion in both Group Ⅱ and group Ⅲ was signifieantly lower than those in the group Ⅰ（P 〈0.05）, which is significantly higher in the group Ⅱ than in the group Ⅲ（P 〈0. 05）. Conclusion Hypothermia can increase the activity of Ca^2 ＋/cabnodulin -dependent kinase Ⅱ after global ischemia reperfusion, Hypothermia has significant protection during isehemia reperfusion injury.

关 键 词：神经细胞保护作用 脑低温 大鼠脑缺血 实验 缺血再灌注损伤 神经细胞损伤 心跳骤停 复苏治疗 治疗性低温 脑保护作用 

分 类 号：R614[医药卫生—麻醉学]