L-天冬门酰胺微生物电极的研制  

Studies on Bacterial Electrode for Determination of L-Asparagine

作　　者：包雅芳 雷呈宏 陈蕾[1] 邓家祺[1] 

机构地区：[1]复旦大学化学系,上海200433

出　　处：《分析化学》1996年第5期535-538,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金;长春应用化学研究所电分析开放实验室资助

摘　　要：以谷氨酸棒状杆菌（ATCC13032）作为生物催化剂，与基础氨气敏电极结合制成对L-天冬门酰胺响应的微生物电极。在30℃时选择pH＝8．5的硼酸缓冲体系，测得电极的线性范围为7．1×10－5～1．1×10－2mol／L，斜率为51．4mV／dec，检测下限为2．0×10－5mol／L，响应时间为4～7min，寿命达30d以上。A bacterial electrode for L-asparagine was prepared by immobilizing the Corynebacterium glutamicum on an ammonia gas sensor. The response of the electrode has a linear range between 7. 1 × 10-5 ~ 1. 1 × 10-2 mol/L L-asparagine with a slope of 51. 4 mV/decade in the pH=8. 5 boric acid-borax buffer at 30℃. The response time is 4~7min. The electrode is relatively stable for 30 days.

关 键 词：谷氨酸棒状杆菌 L-天冬门酰胺 微生物电极 

分 类 号：Q－331[生物学]