高效液相色谱法测定马桑叶中总黄酮含量  

Determination of Flavonoids in Coriaria sinica Maxim Leaf Extracts by HPLC

作　　者：艾国民[1] 张雁冰[1] 王克让[1] 刘宏民[1] 孙建伟[2] 

机构地区：[1]郑州大学新药研究开发中心,郑州450001 [2]河南省农业科学院植物保护研究所,郑州450002

出　　处：《郑州大学学报（理学版）》2006年第1期70-73,87,共5页Journal of Zhengzhou University:Natural Science Edition

基　　金：河南省自然科学基金资助项目;编号0311040900

摘　　要：首次采用反相高效液相色谱法（RP—HPLC）测定了马桑叶中总黄酮含量．马桑叶样品提取物用盐酸水解，以槲皮素为对照品外标法定量，测定了马桑叶提取液中总黄酮含量．采用Shim—pack ODS柱，乙腈-水为流动相，检测波长为360nm．槲皮素进样量在0．0234～2．34μg范围内与其峰面积呈良好线性关系，回归方程为Y（μg）=3．4756×10^-7x＋4．7621×10^-3，r^2=0．99999．加标回收率为98．1％，RSD为2．1％．Quantitative reversed-phase HPLC method is developed for the determination of flavonoids in the extracts of the leaves of Coriaria sinica Maxim. Procedure includes extraction and hydrolysis of the flavonoids and subsequent quantitative chromatographic assay of the obtained aglycones and the free aglycones as well on a Shim-pack ODS column, with acetonitrile-water acetic acid as the mobile phase and detection at 360 nm. The total contents of flavonoids under different circumstances of extraction and hydrolysis are determined by reference to external standard compound quercetin. A good linear relationship of standard is found in the range of 0. 023 4 2.34 μg with good correlation（r^2 =0. 999 99）. Average recovery is 98.1%.

关 键 词：马桑 黄酮 提取物 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]