竹叶中黄酮提取纯化工艺研究  

Extraction and purification technique of flavones from bamboo leaves

作　　者：李瑶[1] 齐晓丽[1] 孟祥颖[1] 鲍永利[1] 乌垠[1] 周毓麟[1] 李玉新[1] 

机构地区：[1]东北师范大学遗传与细胞研究所,吉林长春130024

出　　处：《东北师大学报（自然科学版）》2006年第1期91-94,共4页Journal of Northeast Normal University(Natural Science Edition)

基　　金：国家高技术研究发展计划项目(2004AA2Z3782);国家自然科学基金资助项目(30472169);国家中医药管理局项目(04-05ZP21);吉林省中医药管理局项目(2004-114);吉林省科技发展计划重大项目(20020502)

摘　　要：采用正交试验的方法对竹叶中黄酮的乙醇提取条件进行了系统研究,同时采用大孔吸附树脂吸附法对纯化条件进行了研究.结果表明:以30倍体积80%的乙醇水溶液在80℃水浴中浸提3 h为最佳,4种大孔吸附树脂中AB-8树脂为纯化的最适树脂.Optimal conditions for the extraction of flavones from bamboo leaves were systemically researched by using orthoganol design, and purification conditions were studied by using Macro- reticular resin. The results showed, there were no remarkable difference between abstraction with warm water and ultrasonic technique extraction methods, the optimal conditions for the extraction with warm water bath was 80℃, 30：1 of 80 % ethanol solution to bamboo leaves weight ratio and extracted for 3 hours. From four Macro- reticular resins which all can absorb flavones we finally found that the optimal Macro- reticular resin was AB-8.

关 键 词：竹叶 黄酮 正交设计 大孔吸附树脂 

分 类 号：R284.2[医药卫生—中药学]