RNA聚合酶Ⅱ同BAF复合物和转录因子NF1/CTF之间的联系  

The relation of RNA polymerase Ⅱ and BAF complex with transcription factor NF1/CTF

作　　者：赵丽辉[1] 姜革强[2] 周洪雷[3] 邓国忠[2] 王丽[1] 

机构地区：[1]东北师范大学遗传与细胞研究所 [2]吉林体育学院体育系,吉林长春130022 [3]吉林大学物理学院,吉林长春130012

出　　处：《东北师大学报（自然科学版）》2006年第1期103-107,共5页Journal of Northeast Normal University(Natural Science Edition)

基　　金：吉林省科技发展计划项目(20010710)

摘　　要：通过免疫荧光共定位实验,研究了RNA聚合酶Ⅱ的亚基Rpb1,Rpb6,Rpb8与BAF复合物和转录因子NF1/CTF在基因转录活动中的联系.实验证实,RNA聚合酶Ⅱ的亚基Rpb1与BAF复合物有联系,Rpb6与BAF复合物联系不紧密,Rpb6与NF1/CTF联系密切,Rpb8与BAF复合物和NF1/CTF有部分联系.免疫沉淀实验也证实RNA聚合酶Ⅱ与BAF复合物和NF1/CTF一同参加了转录起始复合物的形成.This paper providest the evidences that the subunit Rpbl, Rpb6, Rpb8 of RNA polymerase Ⅱ have relationship with BAF complex by Immunofluorescene co - orientation assay . Tests validate the sub- unit Rpbl of RNA polymerase Ⅱ have relationship with BAF complex, the small subunit Rpb6 of RNA polymerase Ⅱ have no obvious orientation relationship with BAF complex, then the small subunit Rpb6 of RNA polymerase Ⅱ has obvious orientation relationship with transcription factor NF1/CTF, the small subunit Rpb8 of RNA polymerase Ⅱ have a little orientation relationship with BAF complex and NF1/CTF. The results of Immunoprecipitation assay further demmonstrates RNA polyrnerase Ⅱ and BAF complex with transcription factor NF1/CTF take participate in formation of transcriptional initiation complex together.

关 键 词：BAF复合物 转录因子NF1/CTF RNA聚合酶Ⅱ 

分 类 号：Q813.4[生物学—生物工程]