驱杀菜青虫的内生菌鉴定与化合物研究  

Identification of Endophytic Fungi Killing Pieris rapae and Primary Determination of its Chemical Matter

作　　者：戴传超[1] 余伯阳[1] 王新风[2] 蒋继宏[3] 史央[1] 

机构地区：[1]南京师范大学生命科学学院微生物工程重点实验室 [2]淮阴师范学院生物系,江苏淮安223100 [3]江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《安徽农业科学》2006年第4期694-697,共4页Journal of Anhui Agricultural Sciences

基　　金：江苏省教育厅高新产业项目(JHZD04-006);江苏省药用植物生物技术重点实验室开放项目(KJS03079);淮安市科技局(HAN04048)项目

摘　　要：为了寻找新型驱杀菜青虫的菌种,对大戟科植物内生真菌进行筛选。发现内生菌B6具有很好的驱杀菜青虫的能力,发酵液处理的菜叶对菜青虫校正死亡率达到60.86%。发酵液正丁醇提取物对菜青虫校正死亡率达到42.44%,在薄版层析上呈现3个斑点。发酵液和菌丝均呈浓郁的香味,从菌丝乙酸乙酯提取物中,鉴定出乙酸、乙酸丙酯和3-甲基-2-酮基戊酸甲酯3种成分。对该菌16SrDNAITS序列进行测序,结合形态鉴定确定该菌属于角担子菌属真菌Ceratobasidum stevensii。To find the strains that can expel and control Pieris rapae, the endophytic fungi from the Euphorbiaceae family were screened. The strain B6 showed good expelling and insecticide activity. The adjustment mortality of its fermented broth was 60.86 % in 72 h and the adjustment mortality of n -butanol extract was 42.4.4 %. There were three blots in TLC. There were aromatic smell in the fermented broth and mycelium. 3 - methy-2-oxo-methyester, acetate acid 1 -methylethylester and acetate acid were detected from the mycelium extract of acetate acid ester. There was insecticide activity in the part of n -butanol extract. The strain was identified by rDNA ITS sequences and morphology as Ceratobasidum stevensii. It was benefit to elucidate the eddophytic fungi and the host. The strain would be developed as new insecticide.

关 键 词：内生真菌 驱虫与杀虫 菜青虫 化合物 角担子菌 

分 类 号：Q939[生物学—微生物学]