β-七叶皂甙钠对大鼠脊髓损伤早期脂质过氧化反应和活性氧水平的抑制作用  

Inhibition Effects of Sodium Aescinate on Lipid Peroxidative Reaction and Active Oxygen Species Level in the Early Spinal Cord Injury in Rats

作　　者：陈向阳[1] 郭开今[2] 董启榕[1] 张志明[2] 汤押庚[3] 

机构地区：[1]苏州大学附属第二医院骨科,江苏苏州215004 [2]苏州大学附属第一医院骨科,江苏苏州215006 [3]徐州医学院附属医院骨科,江苏徐州221002

出　　处：《苏州大学学报（医学版）》2006年第1期10-13,共4页Suzhou University Journal of Medical Science

基　　金：江苏省中医药管理局资助课题(9954)

摘　　要：目的观察脊髓损伤后β-七叶皂甙钠(SA)对脊髓损伤(SCI)早期过氧化反应和活性氧水平的抑制作用,探讨SA对脊髓继发性损伤的保护作用。方法SD大鼠72只随机分4组,一组椎板切除不损伤脊髓,另三组采用改良Allen法制备大鼠SCI模型,术后立即分别腹腔注射等体积的生理盐水、SA 5 mg/kg、甲基强的松龙(MP)100 mg/kg。于术后2、12、24 h取SCI段标本,测定损伤段脊髓超氧化物歧化酶(SOD)、脂质过氧化产物丙二醛(MDA)和活性氧水平(ROS)。结果SCI后伤段组织SOD显著减少(P<0.01),MDA和ROS明显升高(P<0.01)。与模型对照组相比,SA能显著提高伤段脊髓组织SOD水平和抑制MDA产生,显著降低ROS水平。其作用和MP相当。结论SA具有抗氧化清除自由基功能,能有效抑制SCI早期受损局部脂质过氧化反应和活性氧水平,对SCI具有保护和治疗作用。Objective To observe the inhibition effects of Sodium Aescinate （SA） on lipid peroxidative reaction and active oxygen species level, and to study the protective effect of SA on early experimental spinal cord injury（SCI） in rats. Methods Seventy two Sprague Dawleys（SD） rats were randomly divided into four groups. Rats in group one（A） had only larninectomy performed and nontraumatized spinal cord samples obtained; rats in the other three groups（group B, C, D） were injured at the level of T10 spinal segment by Allen＇s weight drop method （10 g × 30 mm）, then the normal saline. SA（5 mg/kg） and Methylprednisolone（MP, 100 mg/kg） were given intraperitoneally immediately in group B, group C, group D respectively. At 2, 12, 24 hours following SCI six rats in each group were sacrificed and the injured segments were resected for measuring superoxide dismutase（SOD）, malondialdehyde（MDA） and reactive oxygen species（ROS）. Results Activity of SOD was reduced significantly（P 〈 0.01）, and the level of ROS and MDA were increased significantly （ P 〈 0.01） in the injured spinal cord injury tissue following SCI. SA could obviously prevent reduction of superoxidate dismutase activity（ P 〈 0.01） , reduce the production of MDA（ P 〈 0.01）, and inhabit the ROS level（ P 〈 0.01） in comparison with the SCI model. The effects of SA were the same as that of MP. Conclusion SA may effectively prevent reduction of superoxidate dismutase activity, reduce the production of MDA, and inhabit the ROS level in the injured spinal cord. SA could resist oxidate action and eliminate oxygen free radical of SCI. It plays a great role in protection and treatment of SCI.

关 键 词：Β-七叶皂甙钠 脊髓损伤 自由基 

分 类 号：R651.2[医药卫生—外科学]