小鼠孤雌生殖的胚胎干细胞的初步研究  

A Primary Study of Mouse Parthenogenetic Stem Cell

作　　者：陈林君[1] 刘丽娟[1] 李红霞[2] 王晓琳[1] 沈维干[1] 李朝军[1] 

机构地区：[1]南京师范大学生命科学学院江苏省分子医学生物技术重点实验室,江苏南京210097 [2]南京市妇幼保健院生殖中心,江苏南京210004

出　　处：《南京师大学报（自然科学版）》2006年第1期71-74,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：南京市科学技术局资助项目(200301105)

摘　　要：比较去透明带孤雌桑葚胚和留透明带孤雌囊胚、体内受精胚胎、体外受精胚胎在饲养层上囊胚的扩张率、贴壁生长率.结果表明去透明带孤雌桑葚胚比留透明带孤雌囊胚的扩张率显著(p<0.05)提高,而贴壁率则极其显著地(p<0.01)提高,此外前者还发生增殖.但孤雌胚胎的囊胚扩张率、贴壁率和增殖率均极其显著地(p<0.01)低于体内受精胚胎和体外受精组;体内受精胚胎的体外发育最佳.说明去透明带孤雌桑葚胚比留透明带孤雌囊胚更利于在饲养层上贴壁生长,为建立孤雌生殖的胚胎干细胞系提供了基础.The parthenogenetic morulas with or without zona pellueida are placed onto the feeder layers of PMEFs. The morulas from in vivo fertilization and in vitro fertilization are also cultured under the same condition. Then the rates of expanding and attachment of blastocysts are compared. The rate of expanding from the parthenogenetic morulas without zona pellucida is significantly（p 〈 0.05 ） higher than that of the parthenogenetic blastocysts with intact zona pellueida. The rate of attachment of the former is significantly （p 〈 0.01 ） higher than that of the latter, and moreover, the former has proliferation capability. The rates of expanding ,hatching,attachment and proliferation from the parthenogenetic embryos are significantly （p 〈 0.01 ） lower than those of the other two groups. The embryos from the in vivo fertilization develop best. Therefore, the parthenogenetic morulas without zona pellucida are more capable of attaching onto the feeder layers of PMEFs than the parthenogenetic blastocysts with intact zona pellucida.

关 键 词：体内受精 体外受精 孤雌生殖 卵母细胞 原代小鼠成纤维细胞 

分 类 号：R966[医药卫生—药理学]