毛冠鹿与3种麂属动物的GAPDH基因序列的比较分析  

Analysis on the GAPDH Genes of Muntiacus Muntjak,Muntiacus Crinifrons,Muntiacus Reevesi,and Elaphodus Cephalophus

作　　者：戴君勇[1] 曹祥荣[1] 石磊[1] 张锡然[1] 徐春茂 王强[3] 

机构地区：[1]南京师范大学生命科学学院,江苏南京210097 [2]安徽皖南野生动物救护中心,安徽休宁245400 [3]成都动物园,四川成都610081

出　　处：《南京师大学报（自然科学版）》2006年第1期75-79,共5页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家自然科学基金(39970388);江苏省教育厅自然科学基金资助项目(02KJD180006)

摘　　要：麂亚科（Muntiacinae）动物包括麂属（Muntiacus）和毛冠鹿属（Elaphodus），分布于东南亚、中国及印度，动物形态相似，但具有令人瞩目的细胞遗传学特性．本文以赤麂（Muntiacus muntjak）、黑麂（Muntiacus crinifrons）、小麂（Muntiacus reevesi）和毛冠鹿（Elaphodus cephalophus）4种麂亚科动物为材料，以3-磷酸甘油醛脱氧酶（GAPDH，管家基因）为靶基因，对毛冠鹿在麂亚科中分类地位进行分子鉴定．提取这4种动物的基因组DNA，根据人的GAPDH基因设计引物，进行PCR扩增．获得了约为300bp的扩增产物．将纯化后的扩增产物克隆到质粒pMD18＋TVector中，转化DH-5a菌，挑选阳性克隆。用M13-47／RV—M通用引物进行测序．测序的结果经clustal软件进行了同源性比较．结果显示：麂属动物种问GAPDH基因间的DNA差异在2．52％～4．37％之间，毛冠鹿与麂属3种动物的差异在1．79％-2．87％之间．这些变异以碱基的转换为主．本文结果支持毛冠鹿为独立属1种的分类观念．Muntjac deer （ Muntiacinae, Cervidae）, include Muntiacus and Elaphodus, are distributed throughout Southeast Asia, China, and India. They are of great interest to evolutionary biologists and cytogeneticists because of the considerable diversity of their karyotypes, despite their morphological similarity. The GAPDH gene, a nuclear gene, is analyzed for identifying the taxonomy of the tufted deer （Elaphodus cephalophus）. The genomic DNAs are extracted from the four animals. The primers are designed and synthesized according to the human GAPDH gene. The fragments of GAPDH gene are amplified by using the genomic DNA of the four animals as templates. The fragment sizes of the PCR products （ GAPDH） are about 300 bp. The target bands are recovered and purified. The fragments are ligated with the plasmid of pMD 18-T Vector by the method of direct T-A cloning. The mixtures of ligation were transformed into DH-5α The positive clones are screened by the white/blue colony, and identified by colony PCR and digested by restriction enzyme. The DNA sequences of the recombinant clones are determined by using M13-47/RV-M universal primers and are aligned by the software of clustal. The nucleotide divergence is 2.52% - 4.37% among three species of Muntiacus, 1.79% - 2.87% between Elaphodus and each species of Muntiacus. The majority of varieties are due to the transition of nucleotides. The results are agreeable with morphological taxonomy.

关 键 词：麂亚科 毛冠鹿 3-磷酸甘油醛脱氢酶 

分 类 号：Q953[生物学—动物学]