慢性镉诱导小鼠巨噬细胞的吞噬功能活性抑制研究  

Study on Inhibition of Phagocytic Function Activity in Macrophages of Chronic Cd-induced Mice

作　　者：单潇潇[1] 周娟[1] 陈龙[1] 赵洁明[1] 

机构地区：[1]南京师范大学生命科学学院生化与生物制品研究所,江苏南京210097

出　　处：《南京师大学报（自然科学版）》2006年第1期80-83,共4页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：江苏省重点实验室开放基金资助项目(KJS01072)

摘　　要：选用60只健康雌性ICR小鼠,随机分为对照组、低剂量氯化镉组(L-Cd组)、高剂量氯化镉组(H-Cd组),每组20只.对照组每天给予普通全价饲料,L-Cd和H-Cd组分别饲喂含镉2和10 mg/kg的全价饲料,连续9周.分别在第3、6、9周观察小鼠腹腔巨噬细胞吞噬率和吞噬指数的变化,并检测血浆TNF-α水平.结果显示,在整个实验期内,L-Cd和H-Cd组腹腔巨噬细胞吞噬率明显低于对照组,且随剂量增加和时间的延长而明显下降,而吞噬指数虽较对照组下降但无显著差异;血浆TNF-α水平变化不明显.提示慢性镉染毒诱发小鼠巨噬细胞吞噬功能低下.Sixty healthy female ICR mice are chosen and divided randomly into control group, low dose CdC12 group （L-Cd group） and high dose CdC12 group （H-Cd group）. During nine week experimental period, the common complete diet is orally provided daily in control group, and the mice in L-Cd and H- Cd groups are given the diets with 2 and 10 mg of Cd per kg feed, respectively, Changes of phagocytic rate and phagocytic index of macrophages in abdominal cavity of animals are investigated in the 3 rd , 6 th and 9 th weeks, respectively, with the analysis of plasma TNF-α level. The results show that during the entire experimental period, phagocytic rates in macrophages of L-Cd and H-Cd groups reduce significantly as compared with that of control group, and drop significantly with dosage increase and temporal elongation of Cd exposure. No obvious differences are observed though the phagocytic indice are lower in Cd-exposed groups than in control group. The levels of plasma TNF-α among all groups remain unchanged. It is suggested that chronic cadmium exposure induces the defect of maerophage functional activity in mice .

关 键 词：镉 小鼠 巨噬细胞 肿瘤坏死因子-Α 

分 类 号：Q343.5[生物学—遗传学]