山东花生主栽品种AFLP指纹图谱的构建  

Establishment of AFLP Fingerprinting of Peanut Cultivars in Shandong Province

作　　者：李双铃[1] 任艳[1] 陶海腾[1] 禹山林[1] 袁美[1] 

机构地区：[1]山东省花生研究所,山东青岛266100

出　　处：《花生学报》2006年第1期18-21,共4页Journal of Peanut Science

基　　金：青岛市自然科学基金(03-2-jz-05);国家科技部重大基础研究前期项目(2002ccc03200)

摘　　要：利用MseⅠ和EcoRⅠ酶切和9对引物组合对10个山东花生主栽品种进行AFLP分析,9对引物组合共扩增产生169条带,其中55条为多态性的,其多态性带比率为32.54%。至少两对引物组合的配合如E-ACT/M-CAA//E-AAG/M-CAC和E-ACC/M-CAA//E-AAG/M-CAC可将这10个品种完全区分开。Two restriction endonucleases Mse I and EcoR I and 9 primer combinations were used to AFLP analysis among 10 peanut cultivars. 169 bands were amplified by the 9 primer combinations, 55 among them were polymor phic and percentage of polymorphic band is 32.54 %. The 10 peanut cultivars could be distinguished from each other by using a minimum of two primer combinations, such as E--ACT/M--CAA and E--AAG/M--CAC, E--ACC/M -- CAA and E-- AAG/M-- CAC.

关 键 词：花生 AFLP 指纹图谱 

分 类 号：S828[农业科学—畜牧学] S668.4[农业科学—畜牧兽医]