慢性心力衰竭患者CD4+CD25+调节性T细胞检测及意义  

Significance of the change of CD4+CD25+ regulatory T cells in patients with chronic heart failure

作　　者：程翔[1] 廖玉华[1] 郑金娥[2] 夏春燕[1] 王朝晖[1] 周子华[1] 张家明[1] 陈健[1] 舒砚文[1] 毛小波[1] 袁璟[1] 

机构地区：[1]华中科技大学协和医院心内科华中科技大学同济医学院心血管病研究所,武汉430022 [2]华中科技大学协和医院干细胞中心华中科技大学同济医学院血液病研究所

出　　处：《临床心血管病杂志》2006年第3期145-147,共3页Journal of Clinical Cardiology

基　　金：国家自然科学基金资助(No:30370574)

摘　　要：目的：探讨慢性心力衰竭（CHF）患者外周血CD4＋CD25＋调节性T细胞（Treg）水平及意义。方法：采用流式细胞分析法，检测42例CHF患者（CHF组）和16例正常对照者（对照组）外周血CD4＋CD25＋Treg／CD4＋T细胞比例。结果：CHF患者外周血CD4＋CD25＋Treg／CD4＋T细胞比例[（12．2±3．8）％]显著低于对照组[（16．3±5．2）％]。CD4＋CD25＋Treg／CD4＋T比例在缺血性心脏病者[（12．6±4．1）％]与非缺血性心脏病者（12．0±3．7％）间差异无统计学意义，但心功能NYHA分级Ⅲ～Ⅳ级者[（10．4±3．2）％]比例明显低于Ⅰ～Ⅱ级者[（13．3±3．8）％]。结论：CHF患者外周血Treg比例减少，且与心功能有一定关系。CD4＋CD25＋Treg比例降低可能打破了外周免疫耐受，参与了心力衰竭的发生发展。Objective： To explore the significance of the change of CD4＋CD25＋ regulatory T cells （Treg） in patients with chronic heart failure（CHF）. Method： The frequencies of CD4＋CD25＋ Treg were detected in 42 patients with CHF and 16 healthy donors by flow cytometry. Result： The frequencies of CD4＋CD25＋ Treg were found to be significantly lower in patients with CHF （[12.2±3.8]%）than those of healthy donors （[16.3±5.2]%）. There was no significant difference on the frequencies of CD4＋CD25＋ Treg between ischemic heart disease group and non-isehemic heart disease group. The frequencies of Treg was markedly lower in patients with bad heart function （NYHA Ⅲ～Ⅳ） than those of in patients with good heart function （NYHA Ⅰ～Ⅱ）. Conclusion： The frequencies of Treg decrease in patients with CHF and are associated with heart function. The reduce of Treg may lead to the breakdown of peripheral autoimmune tolerance and participate in the process of CHF.

关 键 词：心力衰竭 CD4+CD25+调节性T细胞 自身免疫 

分 类 号：R541.6[医药卫生—心血管疾病]