艾迪注射液抑制肿瘤细胞生长及诱导肿瘤细胞凋亡的研究  

Effects of AiDi on Hepatocellular-carcinoma Cells Apoptosis

作　　者：唐斓[1] 曾凡波[1] 王涛[1] 吴明珲[1] 

机构地区：[1]华中科技大学同济医学院药学院药物研究室,武汉430030

出　　处：《中国药师》2006年第3期198-200,共3页China Pharmacist

摘　　要：目的:对艾迪注射液体外抗癌活性进行研究,探讨其抗肿瘤作用及机制。方法:应用四甲基偶氮盐(MTT)法检测艾迪注射液在体外对小鼠S180,H22及人HepG2细胞的生长抑制作用;流氏细胞仪(FCM)分析其诱导HepG2细胞凋亡和对周期的阻滞作用;用荧光显微镜观察其诱导HepG2细胞凋亡作用。结果:艾迪注射液对小鼠S180、H22及人HepG2细胞的IC50分别为(9．82±0．272),(4．25±0．333),(6．78±0．268)mg·ml-1。4．1 mg·ml-1艾迪注射液作用48 h后的HepG2细胞凋亡率达到12．43％。结论:艾迪注射液对肿瘤细胞具有直接杀伤和促进肿瘤细胞凋亡的作用。Objective： The anti-cancer activity of AiDi and the related mechanism was studied. Method： The effect of AiDi on tumor cell proliferation, cell cycle distribution and apoptosis inducing were determined by cell biological methods including MTT assay, flow cytometry and fluorescent staining. Result： The IC50 of AiDi on S180 and H22 mice cell lines and HepG2 cell line were （9.82 ± 0.272）, （4.25 ±0.333） and （6.78 ±0.268）mg·ml^-1 respectively, After offering AiDi of 4.1 mg·ml^-1 for 48 hours, the apoptosis rate of HepG2 cells was 12. 43%. Conclusion： AiDi showed proliferation inhibition on Si50 and H22 mice tumor cell lines and HepG2 human hepatocellular-carcinoma cell line by killing tumor cells directly and inducing apoptosis.

关 键 词：艾迪注射液 肿瘤细胞 凋亡 小鼠 

分 类 号：R285.5[医药卫生—中药学]