高效液相色谱法测定人血浆中替米沙坦的浓度  

Determination the Content of Telmisartan in Human Plasma by HPLC

作　　者：陶达人[1] 孙黎[1] 沈金芳[1] 

机构地区：[1]上海第二医科大学附属仁济医院临床药理研究室,上海200001

出　　处：《中国药师》2006年第3期221-222,共2页China Pharmacist

摘　　要：目的:建立HPLC法测定人血浆中替米沙坦浓度。方法:采用Dimak Diamonsil钻石C18色谱柱(200 mm×4．6 mm,5μm);流动相,水-三乙胺-乙腈(73:0．12:27);荧光检测激发波长为305 nm,发射波长为380 nm。结果:替米沙坦线性范围为1 -200 ng·ml-1,最低检测限为0．25 ng·ml-1。结论:该法准确、灵敏、简便。Objective： To establish a HPLC method to determine the content of telmisartan in human plasma. Method： Sepration was achieved on the Dimak Diamonsil C18 column （5μm,200 mm × 4.6 mm）. The mobile phase consisted of Water-Acetonitrile-Triethylamine（73： 0.12：27 ,v/v/v） and was used at the flow rate of 1,0 ml·min^-1. The fluorimetric excitation and emission wavelengths were set at 305 nm and 380 nm. Result： The retention time of telmisartan was about 6min. The calibration curves were linear in range 1 - 200 ng ·ml^-1. The lower limit of quantitation was 0.25 ng·ml^-1. Conclusion： The HPLC method can be used to determine the content of telmisartan in human plasma.

关 键 词：替米沙坦 高效液相色谱法 血药浓度 

分 类 号：R927.2[医药卫生—药学]