四环素调控SV40Tag转基因小鼠模型的建立  

Establishment of Tetracycline-Dependent Regulation of SV40 Tag Transgenic mice

作　　者：孙强[1] 岳挺 刘英[2] 冯洁 董娟 曹慧 章蓉 朱洪 刘全海[2] 李厚达 

机构地区：[1]扬州大学比较医学中心,扬州225009 [2]上海市医药工业研究院药理室,上海200437

出　　处：《中国实验动物学报》2006年第1期8-11,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金："十五"国家科技攻关计划资助项目(批准号:2001BA70113)

摘　　要：目的构建四环素调控的SV40T转基因小鼠模型。方法同时显微注射外源基因p205-rtTA-C3和pTRE-Tag至FVB小鼠原核,注射受精卵移植到同期发情的假孕受体出生个体,经PCR和Southern检测获得阳性转基因小鼠。结果经PCR结合Southern检测得到rtTA和Tag双阳性转基因小鼠一只,rtTA单阳性两只和Tag单阳性一只。结论通过饮水给与四环素的双阳性小鼠可在卵巢中检测到Tag mRNA的表达。Objective To establish a tetracycline-dependent regulation of SV40 Tag transgenic mice. Methods p205- sv40Ta p205-rtTA-C3 and pTRE-Tag dual transgenic mice was generated by pro-nuclear microinjection and embryo transplantation. Positive founder mice were identified through both PCR and Southern blot. Results A rtTA and Tag dual positive transgenic mice, two rtTA single and one Tag single transgenic mice were confirmed by PCR and Southern Blot. The expression of Tag mRNA was detected by RT-PCR in the ovary of the dual transgenic mice which were administered with tetracycline in the drinking water. Conclusion The results of this study indicate that it is possible to establish a tetracycline-dependent regulation of SV40 Tag transgenic mice by administeration of tetracycline in the drinking water.

关 键 词：四环素 基因表达调控系统 转基因 模型 动物 

分 类 号：R349.64[医药卫生—基础医学]