人ZP3融合蛋白的原核表达  

Expression of ZP3 fusion protein in prokaryotic cells

作　　者：郭焱[1] 邹冬辉[1] 左文静[1] 路英丽[1] 李丹地[1] 王忠山[1] 

机构地区：[1]吉林大学基础医学院细胞生物教研室,130021

出　　处：《中国妇幼保健》2006年第6期820-822,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金资助项目(NO.39870313)

摘　　要：目的:在原核中表达人卵透明带蛋白3(zone pelluc ida,ZP3)。方法:将人ZP3基因的核心片段克隆到原核表达载体pGEX-4T-1中。经酶切和序列分析后,用重组质粒转化大肠杆菌BL21,并经丙基β-D硫代半乳糖苷(IPTG)诱导产生GST-ZP3融合蛋白。结果:重组菌株明显诱导表达出预期分子质量为63 000 D的融合蛋白。结论:成功地构建GST-ZP3融合蛋白表达载体,并在大肠杆菌中表达GST-ZP3融合蛋白,为进一步开展免疫避孕与生殖免疫的研究奠定基础。Objective： To express zone pellucida3 （zp3） in prokaryotic cells. Methods： The core fragment of zp3 gene was cloned into plasmid pGEX - 4T - 1 containing glutathiones transferase （GST） fusion protein gene. Following restriction enzyme digestion analysis and sequencing, pGEX -4T-1ZP3 was transformed into E. coli BL21. GST- ZP3 fusion protein was expressed under IPTG induetion. Results： A 63 000 Dalton fusion protein was detected, as expected, was obtained evidently. Conclusion： A prokaryotic system capable of expressing GST - ZP3 fusion protein is successfully constructed. This will facilitate our study of the immunocontraception.

关 键 词：卵透明带蛋白3 融合蛋白 原核表达 

分 类 号：R321.1[医药卫生—人体解剖和组织胚胎学]