饲粮添加锌对蛋鸡金属硫蛋白基因表达的影响  

Diet Supplementation of Zinc Affects MT mRNA Expression in Laying Hens

作　　者：李发弟[1] 张军霞[1] 倪迎冬[2] 赵茹茜[2] 郝正里[1] 郎侠[1] 刘建斌[1] 

机构地区：[1]甘肃农业大学动物科学技术学院,兰州730070 [2]南京农业大学农业部动物生理生化重点开放实验室,南京210095

出　　处：《畜牧兽医学报》2006年第3期305-308,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

摘　　要：选用66只18周龄商品代伊莎蛋鸡,随机分为3组,每组22只。对照组饲喂基础饲粮,试验1、2组在基础饲粮中分别添加60和180 mg/kg锌(以ZnSO4.H2O形式),10周后屠宰采样。采用相对定量反转录多聚酶链式反应(RT-PCR)方法检测不同锌添加水平对蛋鸡肝脏和肾脏中金属硫蛋白(Metallothionein,MT)mRNA表达水平的影响。结果表明:试验1组蛋鸡肝脏中MT mRNA表达丰度显著高于试验2组和对照组(P<0.05),而试验2组与对照组之间无明显差异(P>0.05)。试验1组蛋鸡肾脏MT mRNA表达丰度显著高于试验2组(P<0.05),而试验1、2组与对照组之间均无显著性差异(P>0.05)。在饲粮中添加低水平锌可显著上调蛋鸡肝脏和肾脏中MTmRNA的表达。The present experiment was conducted to determine the changes of hepatic and renal metallothionein mRNAs expression in response to different levels of zinc supplementation in ISA laying hens . The laying hens were divided into the groups of control, treatment 1 （60 mg/kg ） and treatment 2 （180 mg/kg） at 18 weeks of age and sacrificed after 10 weeks for tissue sam piing. The levels of mRNA expression were quantified with RT-PCR, using β-aetin as an internal standard. The results showed that zinc supplementation at 60 mg/kg, but not 180 mg/kg, signif icantly increased the hepatic MT mRNA level compared with the control （P〈0.05）. Additionally, the level of renal MT mRNA expression was significantly higher in 60 mg/kg compared with that in 180 mg/kg group （P〈0. 05）, although no significant differences were observed between control and either of the two treatment groups. The results suggest that lower level of zinc supplementation significantly up-regulate MT gene expression in the liver and kidney of the laying hens.

关 键 词：伊莎蛋鸡 锌 金属硫蛋白 反转录多聚酶链式反应 肝脏 肾脏 

分 类 号：S831.5[农业科学—畜牧学]