脂糖舒对糖尿病大鼠血液T淋巴细胞亚群和免疫器官系数的影响  

Effects of Zhi Tang Shu on the T lymphocyte subgroups in blood and the immuno-organs coefficients of diabetic rats

作　　者：姚旌旗[1] 舒思洁[2] 赵骥[3] 吴基良[2] 

机构地区：[1]咸宁学院免疫学教研室,湖北咸宁437100 [2]咸宁学院药理学教研室 [3]咸宁学院中心实验室

出　　处：《山东医药》2006年第7期8-10,共3页Shandong Medical Journal

基　　金：湖北省教育厅资助课题(2004DO02)

摘　　要：目的探讨脂糖舒对糖尿病大鼠血液T淋巴细胞亚群和免疫器官系数的影响。方法采用链脲霉素30mg/kg腹腔注射1次/3d制备糖尿病大鼠模型,用不同剂量脂糖舒0.5g/kg或1.0g/kg和二甲双胍156.25mg/kg灌胃8周;另取健康大鼠作正常对照。应用流氏细胞仪检测血液T淋巴细胞亚群CD3+、CD+3/CD4+及CD3+/CD+8的百分率,计算CD4+/CD8+比值;然后称体重,处死大鼠,取出胸腺和脾脏称重,计算胸腺和脾脏器官系数。结果1模型组CD3+、CD3+/CD4+和CD3+/CD+8及体重、胸腺系数和脾系数均显著低于正常组(P<0.01),CD4+/CD+8比值显著高于正常组(P<0.05);2脂糖舒-组与模型组比较,除CD4+/CD8+比值无显著性差异(P>0.05)外,其余指标均显著升高(P<0.05);二甲双胍组的脾系数和CD+4/CD8+比值与模型组无显著性差异(P>0.05),其余指标显著升高(P<0.05);3与二甲双胍组比较,脂糖舒-组的体重和胸腺系数显著升高(P<0.05),其余指标无差异(P>0.05);4与脂糖舒-组比较,脂糖舒-组仅胸腺系数显著升高(P<0.05),其余指标无差异(P>0.05)。结论大剂量脂糖舒具有明显的增强糖尿病大鼠细胞免疫作用。Objectivez To investigate the effects of Zhi Tang Shu （ZTS） on the T lymphocyte subgroups in hlood and the immuno-organs coefficients of diabetic rats. Methods.. The diabetic rats model was established by ahdominal injection of small dosage （30mg/kg） of streptozotocin for three times, every three days a time. Metformin hydrochloride tablets （MHT, 156.25mg/kg） and ZTS （0. 5g/kg and 1.0g/kg） were respectively perfused into stomach for 8 weeks, and healthy rats were used as control. Then determined the percents of CD3^＋, CD3^＋/CD4^＋ and CD3^＋/CD8^＋ with flow cytometry. Weighed an killed the rats,then weighed the thymus and spleens. The organs coefficient of thymus and spleens and the ratio of CD4^＋/CD8^＋ were calculated. Results ： ①The percent of CD3^＋, CD3^＋/CD4^＋ and CD3^＋/CD8^＋ and organs coefficient of thymus and spleens and weight of bodies in diabetic model control group were lower than those of normal group （P〈0. 01）. But the ratio of CD4^＋/CD8^＋ was higher than that Of normal group （P〈0.05）; ②The all other indexes of ZTS Ⅱ group were higher than those of diabetic model control group （P〈0. 05） except the ratio of CD4^＋/CD8^＋ （P〉0.05）. And except organs coefficient of spleen and the ratio of CD4^＋/CD8^＋ （P〉0.05）, the other indexes in MHT group were significantly increased compared with diabetic model control group （P〈0. 05） ③ The index of bodies weight and organs coefficient of thymus in ZTS Ⅰ group were significantly increased （P〈0. 05）, but other indexes had no significant difference compared with MHT group （P〉0.05） ;④ Except organs coefficient of thymus （P〈0. 05）, the other indexes of ZTS Ⅱ group had no significant difference compared with that of ZTS- Ⅱ group （P〉0. 05）. Conclusions： Higher dosage of ZTS has obvious effect of increasing cell immunological function of diabetic rats.

关 键 词：脂糖舒 糖尿病 T淋巴细胞亚群 器官系数 

分 类 号：R587.1[医药卫生—内分泌]