泻心汤配方颗粒与标准汤剂的HPLC指纹图谱对照研究  

Comparison of Xiexintang Dispensing Granules and standard decoction with 3D-HPLC fingerprint chromatogram

作　　者：马跃平[1] 刘晓秋[1] 陈发奎[1] 

机构地区：[1]沈阳药科大学,辽宁沈阳110016

出　　处：《中成药》2006年第3期317-321,共5页Chinese Traditional Patent Medicine

摘　　要：目的:考察泻心汤(大黄、黄连、黄芩)配方颗粒剂与标准汤剂主要指标成分相对含量的变化。方法:采用3D-HPLC,甲醇(A)、含0.02 mol/L磷酸二氢钾的0.1%磷酸水(pH=3)(B)梯度洗脱,建立泻心汤配方颗粒剂与标准汤剂的指纹图谱。结果:确定了30个共有峰,得到了以黄芩苷为内标的相对保留时间和相对峰面积值。结论:两者主要指标成分相对含量差异较大,泻心汤配方颗粒剂含量较高。AIM： To study the difference in main chemical composition and content between （Radix et Rhizoma Rhei; Rhizoma Coptidis; Radix Scutellariae） dispensing granules and standard decoction. METHODS： 3D- HPLC was used with MeOH （A） ,0.1% H3 PO4-H2 0 （ include 0.02 mol/L KH2 PO4, pH = 3 ） （ B ） gradient elution, the fingerprint chromatogram of Xiexintang Dispensing Granules and standard decoction were set up. RESULTS： 30 peaks were indicated, the relative retention time and relative peak area were obtained. CONCLUSION： The difference between Xiexintang Dispensing Granules and standard decoction is obvious, the contents of the former are higher.

关 键 词：泻心汤 配方颗粒 标准汤剂 指纹图谱 HPLC 

分 类 号：R927.11[医药卫生—药学]