不同产地穿心莲药材色谱指纹图谱的比较研究  

Chromatographic fingerprints of Andrographis paniculata (Burm.f.) Nees.collected from different places

作　　者：董海娟[1] 张尊建[1] 余静[2] 

机构地区：[1]中国药科大学分析测试中心,江苏南京210009 [2]南京医科大学公共卫生学院,江苏南京210029

出　　处：《中成药》2006年第3期321-324,共4页Chinese Traditional Patent Medicine

基　　金："十五"国家重大科技专项"创新药物和中药现代化"资助项目(2001BA701A56)

摘　　要：目的:建立穿心莲药材的HPLC指纹图谱,为其全面质量评价及控制提供参考。方法:采用HPLC/UV法,测定了10个不同产地的23批穿心莲药材样品。色谱条件:Shimadzu ODS柱,流动相为0.1%甲酸乙腈与0.2%甲酸,梯度洗脱,流速1.0 ml/min,检测波长254 nm,柱温为室温。应用聚类分析、非线性映射和相似度计算等方法对所得指纹图谱进行了定性、定量评价。结果:建立了操作简单、分离度佳、重现性好的穿心莲药材指纹图谱检测方法,不同产地药材指纹图谱具有一定差异。结论:聚类分析、非线性映射和相似度计算等方法可实现对指纹图谱的定性、定量评价,所建立的色谱指纹图谱可用于穿心莲药材的鉴别及质量评价。AIM： To study the HPLC fingerprints and establish a sensitive and specific method for the quality control of Andrographis paniculata （Burm. f. ） Nees. METHODS： All 23 samples of Andrographis paniculata （Burro. f. ） Nees collected from 10 different places were determined by RP-HPLC. Shimadzu-ODS column was used with maxtures of 0. 1% formic acid-acetonitrile and 0. 2% formic acid-water as mobile phase in gradient mode. The flow rate was set at 1.0 mL/min. The column temperature was set at 25℃ and detecting wavelength at 254 nm. Hierarchical clustering, nonlinear mapping and similarity criteria were applied to evaluate the fingerprints. RESULTS： The simple and specific method with good repeatability was established. There were different contents of each component contained in habitat samples produced in individual area. CONCLUSION： The proceeding is suitable to differentiate Andrographis paniculata （ Burro. f. ） Nees from different places conveniently and can be used for the quality control of this herb.

关 键 词：穿心莲 指纹图谱 高效液相色谱 聚类分析 非线性映射 模式识别 

分 类 号：R284.1[医药卫生—中药学]