彩鲫ran基因原核表达蛋白多克隆抗体的制备  

Preparation of Multiclonal Antibody against Expressed Protein of Color Crucian Carp ran Gene in E.coli

作　　者：李常健[1] 蒋琼凤[1] 

机构地区：[1]湖南科技学院生命科学与化学工程系,湖南永州425006

出　　处：《淡水渔业》2006年第2期3-5,共3页Freshwater Fisheries

基　　金：湖南省自然科学基金项目(编号:05JJ40035);湖南省高等学校科研经费(青年项目;编号:04B052)资助

摘　　要：采用SDS-PAGE分离纯化彩鲫(Carassius auratus gibelio)ran基因编码区cDNA全长序列的原核表达蛋白,并以此为抗原免疫家兔,制备了相应的多克隆抗体;W estern b lotting结果表明,该抗体效价为1∶1000,具有较高的特异性。并从抗原蛋白相对分子质量、每次注射蛋白量、注射方式、注射途径等诸方面对该技术的要点进行了介绍。The protein coded by the full - length cDNA sequence of ran gene from color crucian carp （ Carassius auratus gibelio） expressed in E. coli was separated and purified by SDS -PAGE in our study. Moreover, the expressed protein was applied to immunize rabbits and corresponding muhiclonal antibody was prepared. Western blotting results indicated that the prepared antibody had the high titer（ 1 ： 1000） and high specificity. Moreover, the keys of this technique including the relative molecular mass of antigen, the quantity of proteins in each injection, procedure of injection, and approaches of injection were also introduced in this paper.

关 键 词：彩鲫 ran基因 原核表达蛋白 多克隆抗体 

分 类 号：S917[农业科学—水产科学] Q959.403[生物学—动物学]