黄河鲶不同组织中RNA提取纯化方法研究  

The Method of Extracting and Purifying RNA from Different Tissues of the Yellow River Catfish

作　　者：吴旭东[1] 侯玉霞[1] 张文吉[1] 

机构地区：[1]中国农业大学理学院,北京100094

出　　处：《淡水渔业》2006年第2期24-26,共3页Freshwater Fisheries

基　　金：宁夏回族自治区8613重大农业科技攻关项目(2002-Z01-04)资助

摘　　要：从组织提取RNA的完整性对于分子生物学研究是至关重要的。RNA提取方法有多种。采取异硫氰酸胍法从黄河鲶肌肉、血液、全鱼、肾脏、肝脏、卵巢中提取出完整RNA,结果表明,通过该方法所提取RNA质量高,效果好,可以用于进一步的分子生物学研究。The integrality of RNA extracted and purified from tissues is essential for molecular biology. Some useful extracting and purifying methods had been discovered. Guanidine Thiocyanate is ideal for extracting and purifying RNA of the Yellow River catfish. Through this method, RNA of high quality had been obtained from muscle, blood, kidney, liver, ovary, which can be used for the further molecular biology research.

关 键 词：黄河鲶 RNA提取 异硫氰酸胍法 

分 类 号：S917[农业科学—水产科学] Q959.406[生物学—动物学]