柑橘体细胞杂种有性后代叶形态遗传变异研究  

Study on the heredity and variation of leaf morphological character of sexual progenies of the citrus somatic hybrid

作　　者：伊华林[1] 荣融[1] 

机构地区：[1]华中农业大学作物遗传改良国家重点实验室,华中农业大学国家柑橘育种中心,武汉430070

出　　处：《果树学报》2006年第2期169-172,共4页Journal of Fruit Science

基　　金：国家"十五"计划资助项目(编号:2004BA515B09)。

摘　　要：对柑橘体细胞杂种有性后代叶形态遗传变异研究表明,有性后代群体叶长、叶宽、叶面积平均值低于双亲,叶厚与双亲接近,而气孔长、宽与密度平均值介于双亲之间;不同有性后代植株叶长、叶宽、叶面积、叶厚及气孔长、宽与密度变化范围与杂交双亲对应的参数呈交叉现象,二倍体与三倍体群体间没有明显分组。有性后代除叶厚变异范围较小外,其它参数变异范围都较大。经次数资料的适合性测验即χ2测验,叶形、叶尖缺刻的有无及翼叶形状分离符合1∶1的分离规律,二倍体组群与三倍体组群的叶长差异极显著、叶宽差异显著、叶厚没有差异,气孔长与密度差异极显著,而气孔宽差异显著。The heredity and variation of leaf morphological character of sexual progenies of citrus somatic hybrid has been studied. Results showed that the average leaf length, width and area of sexual progenies was smaller than those of their parents and the thickness was similar to that of parents, whereas the average stoma length and density were intermediate between of the two parents. The variation range of leaf length, width, area, and thickness and stoma length, width, density of the sexual progenies were crisscrossed with their parents. The variation range of other parameters was larger, except the leaf thickness. x2（Chi-Square） test indicated that 1 ：1 segregation was detected in leaf shape, presence or absence of leaf apex notch and wing leaf shape. If the diploids and triploids are considered as an independent population respectively, by the hypothesis test of the average notably significant difference was observed in leaf length and stoma length and density between the two populations, together with significant difference in leaf width and stoma width; while the leaf thickness is almost the same. However, there was no obvious gap in leaf length, width, thickens and stoma size, density of single plant between diploids and triploids; their observation value crisscrossed with each other.

关 键 词：柑橘体细胞杂种 有性后代 叶形 遗传变异 

分 类 号：S666[农业科学—果树学]