聚乙烯醇在枣和酸枣组织培养中的作用  

Effects of PVA on tissue culture of Dongzao jujube (Ziziphus jujuba) and Z. acidojujuba

作　　者：王娜[1] 刘孟军[1] 秦子禹[1] 

机构地区：[1]河北农业大学中国枣研究中心,河北保定071001

出　　处：《果树学报》2006年第2期301-303,共3页Journal of Fruit Science

基　　金：科技部科技攻关项目(2001BA502B09-04);河北省科技攻关项目(04220111D)。

摘　　要：以冬枣、酸枣组培苗为试材,研究了聚乙烯醇不同浓度在冬枣和酸枣组培苗继代增殖中对其玻璃化的影响、在生根培养中对生根效果的影响及在叶片培养中对愈伤组织诱导的影响。结果表明,2.0g/L的聚乙烯醇能够有效降低冬枣和酸枣组培苗在继代增殖中的玻璃化现象,并能提高组培苗在生根培养中的生根条数和根长度;1.0、1.5g/L的聚乙烯醇可使酸枣和冬枣组培苗叶片愈伤组织发生时期提前,并可提高愈伤组织发生率和发生量、改善愈伤组织的质量。因此,聚乙烯醇在冬枣和酸枣的继代增殖、生根培养及叶片愈伤组织诱导中均有良好的促进作用。The effects of PVA on tissue culture of Ziziphus jujuba Dongzao and Sour jujube （Z. acidojujuba C. Y. Cheng et M. J. Liu） were studied. The results indicated that 2.0 g/L PVA can reduce the rate of vitrified plantlets of Dongzao and Sour jujube significantly during proliferation and can also improve both the root number of single plantlet and the length of roots. During leaf culture, using leaves from tissue culture plantlets in Dongzao and sour jujube, 1.0 g/L PVA and 1.5 g/L PVA may quicken callus emergence and increase the rate of callus formation and improve the quality of callus. Therefore, PVA plays an important role in proliferation, rooting culture and leaf culture.

关 键 词：冬枣 酸枣 聚乙烯醇 组培苗玻璃化 生根培养 叶片培养 

分 类 号：S665.1[农业科学—果树学]