化学发光定量测定HPL在孕产妇监护中的意义  

Clinical Value on Pregnant Woman Surveillance by Assaying HPL Using Chemiluminescence Immunoassay

作　　者：史巧英 王晓谦 

机构地区：[1]洛阳市口腔医院,河南洛阳471003 [2]洛阳康达药业有限公司,河南洛阳471002

出　　处：《河南科技大学学报（医学版）》2006年第1期22-23,共2页Journal of Henan University of Science & Technology:Medical Science

摘　　要：目的用连续测定HPL的方法判断产妇胎盘功能,诊断胎儿在宫内的生长发育情况。方法通过HPL单克隆抗体作为固相包被,HPL多克隆抗体与辣根过氧化物酶偶联制成酶标抗体,以鲁米诺作为发光底物方式建立的双抗体夹心法人血清HPL化学发光免疫定量分析法。结果孕妇携有先兆流产、异常妊娠等症时,HPL值将连续下降。当HPL<4 mg/L时,流产不可避免。结论采用化学发光免疫分析法定量检测孕妇血清中HPL含量,对快速诊断胎盘功能及胎儿发育迟缓等疾病有一定的临床意义。Objective To test the placenta function of pregnant woman and determine the growth of embryo in uterus. Methods The two-site assay was based on the direct sandwich technique. A monoclonal antibody was employed for coating, another polyclonal antibody for labeling with horseradish peroxidase. Luminol was used for the substrate. Results When pregnant women had such symptoms as threathen abortion and abnormal pregnance, HPL value would continuously decrease. And when HPL value reached 〈 4 mg/L, abortion would inevitably occur. Conclusion It has clinical value on immediately diagnosis of the placenta function by using chemiluminescence immunoassay to test human placental lactogen.

关 键 词：化学发光免疫分析法 人胎盘泌乳素 诊断 

分 类 号：R714.2[医药卫生—妇产科学]