胶体金法快速检测霍乱弧菌诊断试验的研究设计与分析  

Study on diagnostic test of colloidal gold technique for rapid detection of Vibrio cholerae

作　　者：李少彤[1] 栾玉明[1] 郭钜旋[1] 邓志爱[2] 钟豪杰[3] 曾德荣[1] 潘捷云[1] 卢树成[1] 

机构地区：[1]广州市海珠区疾病预防控制中心,广州510288 [2]广州市疾病预防控制中心,广州510080 [3]广东省疾病预防控制中心,广州510300

出　　处：《中国卫生检验杂志》2006年第3期269-271,共3页Chinese Journal of Health Laboratory Technology

基　　金：广州市海珠区科技计划项目(2005-D-18)

摘　　要：目的:评价胶体金法对霍乱弧菌检测的真实性、可靠性和实用性,探讨其应用价值。方法:用血清凝集试验方法作为金标准,并用胶体金方法对同一份标本进行检测,对胶体金方法的诊断试验的评价指标作出计算。结果:两种方法的检测结果之间有极好的一致性(φ=0.943,Kappa=0.941),胶体金法诊断霍乱与金标准方法的差异无统计学意义(P>0.05),胶体金法检测霍乱弧菌诊断试验的灵敏度为93.18%,特异度为100%,阳性预测值为100%,阴性预测值为95.38%,假阴性率为6.82%,假阳性率为0。结论:胶体金法在基层霍乱检测工作中具有一定的应用价值,该方法的特异性高,一致性也较高,但其灵敏度仍有待提高。Objective：To evaluate the validity, reliability, accuracy of colloidal gold technique for detection of Vibrio cholerae, and assess its utility. Methods：The gold standard for detection of Vibrio cholerae is agglutination test. Moreover, the same specimen was also examined with colloidal gold technique, and some indexes which evaluated the diagnostic test of colloidal gold technique were calculated in this paper. Results：The results indicated that the two methods were dependent, and the internal consistency was excellent （φ =0. 943, Kappa =0. 941 ）, the difference between the colloidal gold technique and the gold standard for detection of Vibrio cholerae had no statistical significance （P 〉 0. 05 ）. Sensitivity of the diagnostic test is 93. 18%, specificity is 100%, positive predictive value is 100%, negative predictive value is 95. 38%, false negative rate is 6. 82%, false positive rate is 0. Conclusion ：There were some utilities of the colloidal gold technique for detection of Vibrio cholerae in substrate. Specificity of the test is best, and the results indicated that its internal consistency is excellent, but its sensitivity need to be improved in the future.

关 键 词：胶体金 霍乱弧菌 诊断试验 

分 类 号：R378.3[医药卫生—病原生物学]