短波辐射对HBV转基因小鼠树突状细胞功能的影响  

The effect of short wave diathermy on dendritic cells in hepatitis B virus transgenic mice

作　　者：侯安继[1] 杨占秋[1] 高春华[2] 蒋涵[3] 黄菁[3] 

机构地区：[1]武汉大学医学院病毒所,武汉430071 [2]华中科技大学同济医学院附属同济医院康复医学科 [3]武汉大学中南医院中西医结合科

出　　处：《中华物理医学与康复杂志》2006年第3期162-165,共4页Chinese Journal of Physical Medicine and Rehabilitation

摘　　要：目的通过观察短波辐射对HBV转基因小鼠树突状细胞(DC)分泌白介素-12(IL-12)及T 淋巴细胞分泌IL-10、IFN-γ的影响,从而探讨短波辐射治疗慢性乙型肝炎的可能相关机制。方法选取 HBV转基因小鼠20只,将其随机分为短波组和空白对照组,另取10只健康小鼠作为正常对照组,其中短波组小鼠给予短波辐射。各组小鼠分别于实验进行10 d后取其脾脏分离树突状细胞,经流式细胞仪检测 DC表型,采用ELISA方法检测DC分泌IL-12以及在刺激混合淋巴细胞反应中T淋巴细胞分泌IL-10、IFN- γ的含量变化情况。结果短波组DC分泌IL-12水平较空白对照组显著增高(P<0．01),与正常对照组比较,差异亦有统计学意义(P<0．01);在混合淋巴细胞反应中,短波辐射能显著抑制T淋巴细胞分泌IL-10 (P<0．01),并同时提高IFN-γ水平(P<0．01)。结论短波辐射能显著上调HBV转基因小鼠DC功能,对 HBV所致慢性乙型肝炎的临床治疗具有潜在应用价值。Objective To observe the effect of short wave diathermy on the functioning of dendritic cells （DCs） derived from the spleens of hepatitis B virus （HBV） transgenic mice. Methods HBV transgenic mice were treated with short wave diathermy for 10 days. Dendritic cells were then separated from their spleens and incubated with granulocyte-macrophage colony-stimulating factor （GM-CSF） and interleukin- 4 （ IL- 4 ）. The phenotypes of the DCs were assayed by flow cytometry （ FCM ） , IL-12 released by the DCs and IL-10 and IFN-γ produced by the T cells in a mixed lymphocyte reaction（MLR） were measured using ELISA. Results Short wave diathermy could enhance the DCs＇ phenotype expression, increase IL-12 levels （P 〈 0.01 ） , promote the secretion of IFN-γ, and inhibit the secretion of IL-10 in a MLR （P 〈 0.01 ）. Conclusion Shortwave diathermy can significantly up-regulate DC function, so it might be a potential modality used for the treatment of chronic hepatitis B and hepatocellular carcinoma.

关 键 词：短波 树突状细胞 转基因小鼠 慢性乙型肝炎 短波辐射 

分 类 号：R512.62[医药卫生—内科学]