木荷种源遗传多样性和种源区初步划分  

Genetic Diversity Analysis and Provenance Zone Allocation of Schima superba in China Using RAPD Markers

作　　者：张萍[1] 周志春[1] 金国庆[1] 范辉华[2] 胡红宝 

机构地区：[1]中国林业科学研究院亚热带林业研究所,富阳311400 [2]福建省建瓯市林业科技推广中心,建瓯353100 [3]浙江省淳安县林业局,淳安311700

出　　处：《林业科学》2006年第2期38-42,共5页Scientia Silvae Sinicae

基　　金：浙江省科技厅"十五"攻关重点项目"林种树种结构调整支撑技术研究与示范"(011102166);浙江省科技厅一般项目"木荷高效生物防火和材用优良种源筛选和应用"(2004C32072);福建省林业厅科技项目"木荷地理种源试验";浙江省林业种苗管理总站的资助

摘　　要：利用RAPD分子标记研究我国高效生物防火和优良用材树种木荷主要分布区15个地理种源的遗传多样性和遗传结构。结果显示,木荷具有丰富的遗传多样性,木荷种内平均基因多样度为0.3636。研究发现木荷种源Shannon表型多样性与产地纬度呈显著的负相关,南部种源的遗传多样性显著高于北部种源,从而推测25°N左右的自然分布区可能是木荷的分布中心。木荷有27.14%的遗传变异存在于种源间,而72.86%的遗传变异来自于地理种源内。基于分子水平上的种源聚类,可将木荷分布区划分为3个种源区:北缘种源区(安徽南部和浙江北部)、中部种源区(南岭以北、浙江南部以南)和南部种源区(南岭以南),而南部种源区和中部种源区又都可再分为东部和西部2个种源亚区。Genetic diversity and genetic structure of 15 provenances from main natural range of Schima superba, an effective biological fire-resistance and high-quality timber species in South China, were analyzed using RAPD markers. It was indicated that there existed a high degree of genetic diversity for S. superba. Nei＇s gene diversity was 0. 363 6. Shannon＇s information index of provenances was significantly and negatively related to the latitudes of the seed sources. The southern provenances had higher genetic diversity than the northern ones. The natural distribution area at latitude of approximately 25°N may be the central distribution zones of S. superba. Genetic differentiation among provenances was found to be high, and 27.14% of genetic variation existed among provenances. Based on the Nei＇s unbiased genetic distance, the whole natural range of S. superba might be obviously divided into North provenance zone （ the south of Anhui Province and the north of Zhejiang Province）, Middle provenance zone （the north of Nanling mountain and the south of South Zhejiang） and South provenance zone （the south of Narding mountain）. The South and Middle provenance zone could all be further subdivided into East and West sub-zones.

关 键 词：木荷 RAPD 遗传多样性 遗传分化 种源区划 

分 类 号：S718.46[农业科学—林学] S722.7