细菌抗原微阵列中玻片修饰方法比较  

Comparison of modification methods on glass slides used for antigen microarray

作　　者：刘毅[1] 韩金祥[1] 朱波[1] 黄海燕[1] 

机构地区：[1]山东省医药生物技术研究中心现代医用药物与技术重点实验室卫生部生物技术药物重点实验室

出　　处：《临床检验杂志》2006年第2期113-115,共3页Chinese Journal of Clinical Laboratory Science

基　　金：山东省重点攻关项目基金资助(2004GG2202150)

摘　　要：目的建立检测细菌抗原的微阵列技术,并对4种玻片修饰方法进行比较。方法将玻片分别用多聚赖氨酸、3氨-基丙基三甲氧基硅烷(APTES)、戊二醛-PBS、戊二醛-H2O进行修饰,以大肠埃希菌为抗原,检测4种修饰玻片对抗原的固定效率;并以IgG、IgM为抗原,比较IgG和IgM抗体检测的灵敏度。结果4种修饰方法中赖氨酸修饰玻片对细菌抗原的固定效果较好,变异系数最小。结论赖氨酸修饰玻片法适合制备细菌抗原微阵列。Objective To develop an antigen microarray for detection of bacteria and compare four chemical modification methods of glass substrates. Methods E. coli was used as antigen and the immobilizing efficiencies of the antigen on microarray were compared after glass slides were modified with polylysine ,3-aminoproxyhrimethoxylysilane ,glutaraldehyde-PBS,glutaraldehyde-H2O respectively. The sensitivities of detection on the microarray were studied when IgG and IgM were used as the immobilizing antigen, Results All the four kinds of medification methods exhibited a- bilities for immobilizing proteins and keeping the activity of proteins. The slides modified with polylysine shewed highter efficiencies for antigen immobilization and the lowest coetticient of variation. Conclusion The polylysined slides are suitable for antigen microarray.

关 键 词：抗原微阵列 赖氨酸修饰 氨基修饰 醛基修饰 

分 类 号：R446.6[医药卫生—诊断学]